Abstract: Provided is a method for producing a homozygous non-human organism from a heterozygous non-human organism, which homozygous organism can be crossed to obtain a hybrid, comprising providing a heterozygous starting organism; allowing the organism to produce SDR-0 cells through meiosis, which cells originate from second division restitution; regenerating SDR-0 organisms from the SDR-0 cells; and producing the homozygous organism from the SDR-0 organisms thus obtained. Further provided is a method for producing a hybrid, comprising crossing a first homozygous organism that is produced according to the above method with a second homozygous organism. Also provided is a homozygous non-human organism and hybrid non-human organisms obtainable by these methods. In a preferred embodiment, the organisms are plants.

Abstract: The invention relates to a tomato plant, wherein the fruits of which have an improved shelf-life as compared to the fruits of a wild type tomato plant, wherein the genetic determinant causative of the improved shelf life trait is a mutation in the hp2 gene. The increased shelf-life may comprise a fruit that shows normal ripening having a fruit firmness at red ripe harvest that is increased by at least 31%, preferably by at least 42%, more preferably by at least 52%, even more preferably by at least 60%, most preferably by at least 70% as compared to a fruit having similar genetic background that lacks the trait of the invention.

Abstract: The invention relates to a method for testing a plant or plant part for exhibiting a reduced or absent wound-induced surface discoloration as compared to a control plant or plant part, which method comprises providing a plant or plant part that is obtained from a plant population that shows genetic variation, in particular a gene bank; optionally creating a wound surface on the plant or plant part; incubating the plant or plant part or the wound surfaces created thereon to allow for discoloration to occur therein or thereon; observing the discoloration in or on the plants or plant parts; comparing the observed discoloration with the discoloration that is observed in the control plant or plant part to assess whether the plant or plant part shows no discoloration or a discoloration that is reduced as compared to the control plant or plant part; and identifying a plant or plant part that shows no discoloration or a discoloration that is reduced as compared to the control plant or plant part as a plant that harbo

Abstract: Provided is a method for screening a population of plants or plant parts for the presence of individuals showing reduced discolouration compared to a control plant or plant part. The method comprises providing a population of plants or plant parts, optionally creating a wound surface, and incubating the plant, plant parts, or wound surfaces created thereon to allow for discolouration. The discolouration is compared to that of control plants, plant parts, or wound surfaces, and plants or plant parts showing no discolouration or reduced discolouration, compared to control plants or plant parts are identified. Suitably, the discolouration is wound-induced discolouration.

Abstract: The present invention relates to a pepper plant showing the trait fruit formation in the absence of fertilisation wherein said trait is obtainable by introgression from a plant grown from seed that was deposited with the NCIMB under accession number NCIMB 41678, NCIMB 41680, or NCIMB 41684. The invention further relates to progeny of the plant, propagation material for producing the plant, to fruits of the plant and to a food product comprising the fruit or parts thereof.

Abstract: The invention relates to a method for the production of a set of seeds which are genetically identical to the male gametes from which they arise, which may comprise placing a limited number of paternal gametes that have the form of tetrads or dyads on the stigma of a flower to fertilize maternal egg cells to obtain a number of zygotes; and inducing the loss of maternal chromosomes from the zygotes to obtain a seed set containing a limited number of seeds in which the maternal chromosomes are absent. In a preferred embodiment the father plant exhibits suppression of chromosome recombination or second division restitution (SDR) during meiosis.

Abstract: The invention relates to a tomato plant which may comprise the trait fertilization independent fruit formation wherein said trait may be obtainable by introgression from a plant, representative seed of which was deposited with the NCIMB under accession number NCIMB 41626, NCIMB 41627, NCIMB 41628, NCIMB 41629, NCIMB 41630 or NCIMB 41631. Such tomato plant may be obtainable by crossing plants, representative seed of which was deposited with the NCIMB under accession number NCIMB 41626, NCIMB 41627, NCIMB 41628, NCIMB 41629, NCIMB 41630 or NCIMB 41631, with a plant not showing the trait to obtain an FI population; selfing plants from the FI population to obtain an F2 population; preventing pollination of the F2 plants and allowing fruit formation to occur; and selecting plants producing fruits as plants showing fertilization independent fruit formation. The invention further relates to the parthenocarpic fruits, to seeds of the plants and to propagation material of the plant.

Abstract: Provided is a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene-sensitive control under ethylene, are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. Also provided are plants thus selected.

Abstract: The invention relates to a tomato plant the fruits of which have an improved shelf-life as compared to the fruits of a wild type tomato plant, obtainable by introgressing the increased shelf life trait from the mutant LePQ58 (deposit accession number NCIMB 41531) into a tomato plant with a normal shelf-life. The increased shelf-life may comprise a fruit that shows normal ripening having a fruit firmness at red ripe harvest that is increased by at least 31%, preferably by at least 42%, more preferably by at least 52%, even more preferably by at least 60%, most preferably by at least 70% as compared to a fruit having similar genetic background that lacks the trait of the invention.

Abstract: A method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes, wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.

Abstract: Provided is a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene-sensitive control under ethylene, are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. Also provided are plants thus selected.

Abstract: The invention relates to tomato plants which may comprise the trait fertilization independent fruit formation, which may be obtainable by introgression from a plant, representative seed of which was deposited with the NCIMB under accession number NCIMB 41626, NCIMB 41627, NCIMB 41628, NCIMB 41629, NCIMB 41630 or NCIMB 41631. Such tomato plant may be obtainable by crossing plants, representative seed of which was deposited with the NCIMB under accession number NCIMB 41626, NCIMB 41627, NCIMB 41628, NCIMB 41629, NCIMB 41630 or NCIMB 41631, with a plant not showing the trait to obtain an F1 population; selfing plants from the F1 population to obtain an F2 population; preventing pollination of the F2 plants and allowing fruit formation to occur; and selecting plants producing fruits as plants showing fertilization independent fruit formation. The invention further relates to parthenocarpic fruits, seeds of the plants and propagation material of the plant.

Abstract: A method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes, wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.

Abstract: Provided is a method for obtaining DNA from a plant by collecting the root border cells from a growing root and extracting DNA from the root border cells. Preferably, the root border cells are contained in the root exudate of the growing root, which is growing in a medium, for example, water, tissue culture medium, or soil. Suitably, the root is part of a germinating seed, or the root of a seedling, or the adventitious root of a tissue culture plant or plant part.

Abstract: The invention relates to a cucumber (Cucumis sativus) plant, which has the improved shelf life as found in plants and fruits grown from seeds of cucumber EX5001 representative seeds of which were deposited under NCIMB accession number 41670. The plant may be obtainable by crossing a cucumber plant with a plant grown from seeds of cucumber EX5001 representative seeds of which were deposited under NCIMB accession number 41670 and selecting in the F2 progeny of the cross that may be obtained after selfing the F1 for plants showing an improved shelf life.

Abstract: The present invention relates to a for obtaining a plant showing a reduced susceptibility towards infection with a pathogen, in particular an oomycete, comprising treating MO seeds of a plant species to be modified with a mutagenic agent to obtain M1 seeds and growing plants therefrom the thus obtained M1 seeds to obtain M1 plants, inoculation of the thus obtained M1+n plants with the pathogen and selecting plants that show a reduction or absence of sporulation of the pathogen as plants having a reduced susceptibility phenotype. The invention further relates to plants, seeds, pollen, cells and tissues that have the reduced susceptibility towards oomycetes.

Abstract: Provided is a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene-sensitive control under ethylene, are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. Also provided are plants thus selected.

Abstract: Provided is a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene-sensitive control under ethylene, are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. Also provided are plants thus selected.

Abstract: The invention relates to a method for testing a plant or plant part for exhibiting a reduced or absent wound-induced surface discoloration as compared to a control plant or plant part, which method comprises providing a plant or plant part that is obtained from a plant population that shows genetic variation, in particular a gene bank; optionally creating a wound surface on the plant or plant part; incubating the plant or plant part or the wound surfaces created thereon to allow for discoloration to occur therein or thereon; observing the discoloration in or on the plants or plant parts; comparing the observed discoloration with the discoloration that is observed in the control plant or plant part to assess whether the plant or plant part shows no discoloration or a discoloration that is reduced as compared to the control plant or plant part; and identifying a plant or plant part that shows no discoloration or a discoloration that is reduced as compared to the control plant or plant part as a plant that harbo

Abstract: Provided is a method for obtaining DNA from a plant by collecting the root border cells from a growing root and extracting DNA from the root border cells. Preferably, the root border cells are contained in the root exudate of the growing root, which is growing in a medium, for example, water, tissue culture medium, or soil. Suitably, the root is part of a germinating seed, or the root of a seedling, or the adventitious root of a tissue culture plant or plant part.