Abstract: The present invention relates to a method for the identification of antifungal substrates, in particular substrates which are capable of combating filamentous fungi by disturbing cell wall biogenesis. A nucleotide sequence encoding ?-1,3-glucan synthase including its promoter sequences is provided, obtainable from Aspergillus niger, which can be used to develop a reporter system for the identification of a new antifungal compounds in filamentous fungi. The invention relates also to antifungal substrates so obtained and to methods for applying such substrates, in particular in the treatment or prophylaxis of human and animal fungal infections, plant diseases caused by fungi or in the preservation of food against deterioration by fungal growth.

Abstract: An expression system for producing a protein in a filamentous fungus, consisting of a) a host organism selected from the species Trametes and Polyporus and b) a DNS vector containing a selection marker gene. This selection marker gene code is a protein which allows the selection of positive transformants after the transformation of the host organism and which is selected from the group of: antibiotics-resistant genes coding for proteins which eliminate the growth-inhibitory effect of antibiotics against which the host organism is not resistant. Genes coding proteins which are capable of a color-causing reaction; and genes complementing a genetic defect of the host organism (auxotrophy), the expression of the selection marker gene is controlled by at least one active genetic regulation element in the host organism, and c) a DNS vector containing a gene which codes the protein to be produced.