Patents by Inventor Cornell Allen

Cornell Allen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190247436
    Abstract: Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: transfecting the cells by electroporation with a composition comprising (a) a DNA oligo; (b) a DNA digesting agent; and (c) a targeting RNA, wherein the targeting RNA is capped and/or polyadenylated; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.
    Type: Application
    Filed: July 21, 2017
    Publication date: August 15, 2019
    Inventors: Linhong LI, Cornell ALLEN, Madhusudan PESHWA
  • Publication number: 20070059833
    Abstract: The present invention concerns methods and compositions for improving viability and transgene expression in transfected cells. In one embodiment, the present invention provides a method for increasing the viability of a transfected cell, the method comprising: transfecting a cell with a nucleic acid sequence; and contacting the transfected cell with a nuclease in a manner effective to enhance the viability of the transfected cell.
    Type: Application
    Filed: September 7, 2006
    Publication date: March 15, 2007
    Inventors: Linhong Li, Linda Liu, Cornell Allen, Rama Shivakumar, James Brady
  • Publication number: 20060205081
    Abstract: The present invention concerns enhancing transgene expression in electroporated cells. Electroporation-mediated transfection of cells has several advantages over other transfection methods. However, the efficiency of transgene expression in electroporated cells may be lower than desired for certain applications. The ability to further enhance transgene expression in electroporated cells would be useful in a number of applications including protein and virus production. The present invention provides a method for enhancing transgene expression in a cell, the method comprising transfecting the cell by electroporation with an expression construct encoding a protein; and contacting the cell with a methyltransferase inhibitor, wherein the expression of the protein is enhanced as compared to the expression of the protein in a second cell not contacted with the methyltransferase inhibitor.
    Type: Application
    Filed: February 17, 2006
    Publication date: September 14, 2006
    Inventors: Linhong Li, Cornell Allen