Patents by Inventor Craig A. Robin
Craig A. Robin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10563206Abstract: Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.Type: GrantFiled: January 24, 2019Date of Patent: February 18, 2020Assignee: INNOVATIVE TARGETING SOLUTIONS INC.Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Patent number: 10496806Abstract: In a computing device, when a user requests to carry out an operation, the device determines the type of operation requested and the time period since the user was last authenticated. The operation is enabled only if the determined time period does not exceed a threshold for the requested operation.Type: GrantFiled: October 8, 2018Date of Patent: December 3, 2019Assignee: NOKIA TECHNOLOGIES OYInventors: Craig Robin Paskett Heath, Leon Clarke
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Publication number: 20190359937Abstract: A receptor is provided having a heterologous binding site that activates, when bound, a signaling domain related to the TNF receptor superfamily. Methods/uses of the foregoing in whole cell biosensors are also provided. There is also provided a library comprising a plurality of unique biosensor cells for binding unknown binding substrates. Each unique biosensor is a host cell having a receptor which signals production of a positive selectable marker and/or a negative selectable marker in response to the receptor being bound. Also provided is a method of identifying biosensor cells from a library that is specifically activated by a target, involving (a) contacting the library with the target substrate under positive selection conditions; (b) contacting the library with a control substrate under negative selection conditions; and (c) identifying biosensor cells which survive (a) and (b) as biosensor cells which are specifically activated by the target.Type: ApplicationFiled: November 17, 2017Publication date: November 28, 2019Applicant: Innovative Targeting Solutions Inc.Inventors: Craig Robin Pigott, Michael Lajos Gallo, Jaspal Singh Kang
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Publication number: 20190255186Abstract: There is provided a single variable domain T-cell receptor (svd-TCR) comprising a first TCR variable domain, the first TCR variable domain specifically binding to an epitope, that is not a superantigen, in the absence of a second TCR variable domain. Also provided are compositions and cells comprising the svd-TCR as well as methods of identifying the svd-TCR.Type: ApplicationFiled: December 2, 2016Publication date: August 22, 2019Inventors: Michael Lajos GALLO, Jaspal Singh KANG, Craig Robin PIGOTT, Abby LIN, Falene CHAI
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Publication number: 20190144872Abstract: Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.Type: ApplicationFiled: January 24, 2019Publication date: May 16, 2019Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Patent number: 10233453Abstract: Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.Type: GrantFiled: November 2, 2017Date of Patent: March 19, 2019Assignee: INNOVATIVE TARGETING SOLUTIONSInventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Publication number: 20190073463Abstract: In a computing device, when a user requests to carry out an operation, the device determines the type of operation requested and the time period since the user was last authenticated. The operation is enabled only if the determined time period does not exceed a threshold for the requested operation.Type: ApplicationFiled: October 8, 2018Publication date: March 7, 2019Applicant: NOKIA TECHNOLOGIES OYInventors: Craig Robin Paskett HEATH, Leon CLARKE
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Patent number: 10095852Abstract: In a computing device, when a user requests to carry out an operation, the device determines the type of operation requested and the time period since the user was last authenticated. The operation is enabled only if the determined time period does not exceed a threshold for the requested operation.Type: GrantFiled: October 13, 2016Date of Patent: October 9, 2018Assignee: Nokia Technologies OyInventors: Craig Robin Paskett Heath, Leon Clarke
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Publication number: 20180119155Abstract: Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.Type: ApplicationFiled: November 2, 2017Publication date: May 3, 2018Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Patent number: 9914929Abstract: Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.Type: GrantFiled: March 14, 2013Date of Patent: March 13, 2018Assignee: Innovative Targeting Solutions Inc.Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Publication number: 20170334970Abstract: Methods of generating sequence diversity in a protein, such as a ligand-binding protein, are provided. The methods comprise targeted introduction of two or more recombination signal sequences (RSSs) into the protein coding sequence and introduction of the modified protein coding sequence into a recombination-competent host cell, specifically a recombination-competent host cell that is capable of expressing at least RAG-1 and RAG-2, thereby allowing for recombination of the protein coding sequence and expression of variant proteins. Also provided are polynucleotides comprising a nucleic acid sequence encoding a target protein, such as a ligand-binding protein, and comprising two or more RSSs, and compositions and host cells comprising same.Type: ApplicationFiled: May 1, 2017Publication date: November 23, 2017Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott, Abby Lin
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Patent number: 9718871Abstract: Methods of generating sequence diversity in a protein, such as a ligand-binding protein, are provided. The methods comprise targeted introduction of two or more recombination signal sequences (RSSs) into the protein coding sequence and introduction of the modified protein coding sequence into a recombination-competent host cell, specifically a recombination-competent host cell that is capable of expressing at least RAG-1 and RAG-2, thereby allowing for recombination of the protein coding sequence and expression of variant proteins. Also provided are polynucleotides comprising a nucleic acid sequence encoding a target protein, such as a ligand-binding protein, and comprising two or more RSSs, and compositions and host cells comprising same.Type: GrantFiled: March 14, 2013Date of Patent: August 1, 2017Assignee: INNOVATIVE TARGETING SOLUTIONS INC.Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott, Abby Lin
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Publication number: 20170140139Abstract: In a computing device, when a user requests to carry out an operation, the device determines the type of operation requested and the time period since the user was last authenticated. The operation is enabled only if the determined time period does not exceed a threshold for the requested operation.Type: ApplicationFiled: October 13, 2016Publication date: May 18, 2017Inventors: Craig Robin Paskett Heath, Leon Clarke
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Publication number: 20160297883Abstract: Peptide-grafted antibodies having agonist activity against a Class B GPCR and their uses, and methods for identifying same. Mammalian cell lines for high-throughput screening for agonists of a Class B GPCR are also provided that comprise a stably integrated recombinant nucleic acid construct comprising a reporter gene under control of a cAMP responsive promoter, the reporter gene encoding a cell surface expressed protein, and a stably integrated recombinant nucleic acid construct comprising a sequence encoding a Class B GPCR under control of a constitutive promoter.Type: ApplicationFiled: December 4, 2014Publication date: October 13, 2016Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott, Abby YU Chen Lin
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Publication number: 20150329850Abstract: Methods of generating sequence diversity in a protein, such as a ligand-binding protein, are provided. The methods comprise targeted introduction of two or more recombination signal sequences (RSSs) into the protein coding sequence and introduction of the modified protein coding sequence into a recombination-competent host cell, specifically a recombination-competent host cell that is capable of expressing at least RAG-1 and RAG-2, thereby allowing for recombination of the protein coding sequence and expression of variant proteins. Also provided are polynucleotides comprising a nucleic acid sequence encoding a target protein, such as a ligand-binding protein, and comprising two or more RSSs, and compositions and host cells comprising same.Type: ApplicationFiled: March 14, 2013Publication date: November 19, 2015Inventors: Michael Gallo, Jaspal Singh KANG, Craig Robin Pigott, Abby Lin
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Publication number: 20150240243Abstract: Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.Type: ApplicationFiled: March 14, 2013Publication date: August 27, 2015Applicant: Innovative Targeting Solutions Inc.Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Patent number: 8617845Abstract: Compositions and methods are disclosed for generating immunoglobulin structural diversity in vitro, and in particular, for reducing biases in V region and J segment gene utilization, and for generating immunoglobulin V-D-J recombination events in a manner that does not require D-J recombination to precede V-DJ recombination. Selection of advantageous combinations of immunoglobulin gene elements, including introduction of artificial diversity (D) segment genes and optimization of recombination signal sequence (RSS) efficiency, are disclosed.Type: GrantFiled: August 8, 2011Date of Patent: December 31, 2013Assignee: Innovative Targeting Solutions, Inc.Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Publication number: 20130236931Abstract: An in vitro system for generating sequence, and thus structural, diversity in proteins is described. The system can be constructed using appropriately selected nucleic acid molecules that encode regions of a selected protein or proteins and recombination signal sequences (RSS). The selected protein(s) can be, for example, immunoglobulin (Ig) V, D, J and/or C regions, regions of a non-immunoglobulin (non-Ig) protein, or a combination of Ig regions and non-Ig regions. Assembly of such appropriately selected components and their introduction into suitable recombination-competent host cells allows for recombination between the RSS sequences and introduction of sequence and structural diversity into the protein(s).Type: ApplicationFiled: February 12, 2013Publication date: September 12, 2013Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Publication number: 20120258495Abstract: Compositions and methods are disclosed for generating immunoglobulin structural diversity in vitro, and in particular, for reducing biases in V region and J segment gene utilization, and for generating immunoglobulin V-D-J recombination events in a manner that does not require D-J recombination to precede V-DJ recombination. Selection of advantageous combinations of immunoglobulin gene elements, including introduction of artificial diversity (D) segment genes and optimization of recombination signal sequence (RSS) efficiency, are disclosed.Type: ApplicationFiled: August 8, 2011Publication date: October 11, 2012Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott
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Patent number: 8012714Abstract: Compositions and methods are disclosed for generating immunoglobulin structural diversity in vitro, and in particular, for reducing biases in V region and J segment gene utilization, and for generating immunoglobulin V-D-J recombination events in a manner that does not require D-J recombination to precede V-DJ recombination. Selection of advantageous combinations of immunoglobulin gene elements, including introduction of artificial diversity (D) segment genes and optimization of recombination signal sequence (RSS) efficiency, are disclosed.Type: GrantFiled: April 14, 2009Date of Patent: September 6, 2011Assignee: Innovative Targeting Solutions, Inc.Inventors: Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott