Abstract: Vibrio vulnificus can cause infections in aquaculture-raised fish and is considered an opportunistic human pathogen. We isolated V. vulnificus from diseased hybrid tilapia (Oreochromis niloticus X O. aureus) cultured in a North American water reuse aquaculture facility. We have characterized the isolate using biochemical and molecular methods, developed a disease infection model, and determined that formalin-inactivated whole-cell vaccine provides protection against V. vulnificus. The V. vulnificus isolate was determined to be biotype 1, 16S rRNA type B, vcg type C, and vvhA type 2. Fish vaccinated with the formalin-inactivated whole-cell vaccine responded to vaccination as measured by agglutinating antibody titer. In two separate trials, vaccinated tilapia exhibited relative percent survival of 73 and 60% following challenge with the homologous isolate. In additional trials, vaccinated tilapia exhibited survival values of up to 87.5% following challenge with a heterologous isolate.

Abstract: Vibrio vulnificus can cause infections in aquaculture-raised fish and is considered an opportunistic human pathogen. We isolated V. vulnificus from diseased hybrid tilapia (Oreochromis niloticus X O. aureus) cultured in a North American water reuse aquaculture facility. We have characterized the isolate using biochemical and molecular methods, developed a disease infection model, and determined that formalin-inactivated whole-cell vaccine provides protection against V. vulnificus. The V. vulnificus isolate was determined to be biotype 1, 16S rRNA type B, vcg type C, and vvhA type 2. Fish vaccinated with the formalin-inactivated whole-cell vaccine responded to vaccination as measured by agglutinating antibody titer. In two separate trials, vaccinated tilapia exhibited relative percent survival of 73 and 60% following challenge with the homologous isolate. In additional trials, vaccinated tilapia exhibited survival values of up to 87.5% following challenge with a heterologous isolate.

Abstract: Safe and effective live vaccines against Aeromonas hydrophila of fish were created through the induction of rifampicin resistance in native Aeromonas hydrophila isolates; these including rifampicin-resistant mutants NRRL-B-50040 and NRRL-B-50041. Single immersion exposure of fish stimulated acquired immunity against virulent Aeromonas hydrophila infection.

Abstract: Safe and effective live vaccines against Aeromonas hydrophila of fish were created through the induction of rifampicin resistance in native Aeromonas hydrophila isolates; these including rifampicin-resistant mutants NRRL-B-50040 and NRRL-B-50041. Single immersion exposure of fish stimulated acquired immunity against virulent Aeromonas hydrophila infection.

Abstract: A composition prepared from intact killed cells of isolated ?-hemolytic Streptococcus agalactiae and a concentrated extract of a culture of ?-hemolytic Streptococcus agalactiae, is effective for the protection of fish against infection by the same and other virulent strains Streptococcus agalactiae.

Abstract: A safe and effective live vaccine against Edwardsiella tarda in fish was created through the induction of rifampicin resistance in a native Edwardsiella tarda isolate. Single immersion exposure or injection of fish stimulated acquired immunity against virulent E. tarda infection.

Abstract: Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: A safe and effective live vaccine against Flavobacterium columnare of fish was created through the induction of an adhesion deficient isolate of Flavobacterium columnare using a ?-lactam antibiotic (ampicillin). Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: Safe and effective mono and polyvalent vaccines against Streptococcus iniae may be prepared from formalin-killed cells and concentrated extracellular products of Streptococcus iniae which include one or more of deposited strains NRRL B-30238 and NRRL B-30242. Intraperitoneal and intramuscular vaccination of tilapia show acquisition of effective immunity against homologous and heterologous isolates of S. iniae.

Abstract: Methods for the safe and effective live in ovo vaccination of eyed eggs of catfish against enteric septicemia of catfish (ESC) were created through the use of rifampicin resistant native E. ictaluri isolates; these including rifampicin-resistant strain ATCC 202058 of Edwardsiella ictaluri originally isolated from the walking catfish Clarius batrachus from Thailand. Single immersion exposure of eyed eggs of catfish stimulated strong acquired immunity against many isolates of E. ictaluri without the need for booster immunization.

Abstract: Safe and effective live vaccines against enteric septicemia of catfish (ESC) were created through the induction of rifampicin resistance in native E. ictaluri isolates; these including rifampicin-resistant strain (RE-33) of Edwardsiella ictaluri originally isolated from the walking catfish Clarius batrachus from Thailand. Single immersion exposure of catfish fry stimulated strong acquired immunity against many isolates of E. ictaluri without the need for booster immunization.

Abstract: An improved method for diagnosing myofibrogranuloma (MFG) in walleye without harming the fish is described. Blood samples are drawn from walleye and creatinine and calcium levels are then determined along with the sex and length of the fish. These data are then analyzed and compared to data from healthy walleye using discriminant analysis. The process is effective for identifying approximately 88% of MFG-positive walleye.