Abstract: Fusion proteins having a tumor necrosis factor receptor (TNF-R) polypeptide and at least one additional polypeptide covalently fused thereto and selected from an interleukin-1 receptor (IL-1R) and a second TNF-R polypeptide. The receptor polypeptides are preferably fused together by a peptidyl linker. Suitable fusions include, for example, TNF-R-linker-TNF-R; TNF-R-linker-IL-1R; and TNF-R-linker-TNF-R-linker-IL-1R molecules.

Abstract: A dielectric connector for use in high voltage devices, including x-ray tubes, is disclosed. The connector comprises a dielectric material and is pre-formed before attachment to the x-ray tube. Pre-formation of the connector creates a first cavity portion therein that conforms in shape to a corresponding segment of the tube surface. A second cavity portion is also defined for receiving a high voltage receptacle. Upon attachment to the tube, the first cavity portion receives the corresponding tube segment. The high voltage receptacle is received into the second cavity portion and electrically connects with a receptacle defined on the tube surface. The receptacle enables a high voltage signal passing through the electrode to connect with either the anode or cathode disposed within the tube. Pre-formation of the connector enables connector testing and repair to occur before it is attached to the tube, saving resources, time, and cost.

Abstract: Novel 4-1BB ligand (4-1BB-L) polypeptides and a human cell surface receptor designated 4-1BB that binds 4-1BB-L are provided. Isolated 4-1BB-L-encoding and human 4-1BB-encoding DNA sequences, recombinant expression vectors comprising the isolated DNA sequences, and host cells transformed with the recombinant expression vectors are disclosed, along with methods for producing the novel polypeptides by cultivating such transformed host cells. Soluble forms of the 4-1BB-L or 4-1BB polypeptides are derived from the extracellular domains thereof.

Abstract: A method for detecting a target biomolecule directly in a polyacrylamide gel in which it has been separated from other substances. Prior to binding the target to a probe and while the target biomolecule remains in the gel, the gel is immersed in a water miscible aqueous extracting medium containing a base to shrink the gel by at least about ten percent, and then the gel is washed with water to restore the gel to substantially its original size.

Abstract: Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.

Abstract: A method for making ?-ketophosphonates by reacting a trialkyl phosphonocarboxylate (such as triethyl phosphonoacetate) with an acyl halide (such as acetyl chloride) in a reaction involving ester formation, followed by decarboxylation, followed by purification. The reaction is run in a single solvent, which is not removed from the reaction mixture until the purification stage. Suitable solvents for this purpose include halogenated aromatic hydrocarbons (such as mono-chlorobenzene).

Abstract: A needle tip protective device for use with percutaneous entry needles. In one embodiment, the needle tip protective device includes a needle guard slidably mounted on a hypodermic needle, the latter having a needle tip located at the distal end thereof, and a change of profile formed medially there along. The needle guard is movable along the hypodermic needle and engageable with the change in profile formed thereon. The engagement with the change in profile is configured to correspond with the ability of the needle trap to entrap the needle tip once the needle trap has advanced the sufficient distance distally along the hypodermic needle. In further refinements, the needle trap may be biased toward the distal needle tip of the needle.

Abstract: A carpenter's pencil sharpener is designed for cutting a point on a carpenter's pencil that defines a longitudinal axis and has a maximum width or dimension (MD) perpendicular to the longitudinal axis. The sharpener has a contouring portion defining a rotational axis, a pencil point contour surface and a blade. The sharpener also has a centering portion at least rotationally fixed to the contouring portion. The centering portion has a non-rotating, cylindrical, inner surface and an outer end defining an aperture for receiving the carpenter's pencil. The cylindrical surface has an inner diameter (ID) dimensioned to fit the maximum dimension (MD) of the carpenter's pencil, and is large enough to permit rotation of the carpenter's pencil within the cylindrical surface. The centering portion also has a length (L) generally parallel to the rotational axis and measured from the contouring portion to the outer end for maintaining the carpenter's pencil parallel to the axis.

Abstract: A novel air intake system which is suitable for at least substantially reducing the inadvertent intake of water with ambient air into an air compressor or other operational component of a fuel cell or internal combustion engine is disclosed. The air intake system typically includes an elongated air flow conduit having a downwardly-angled air inlet arm that is typically fitted with multiple louver elements and a splash shield to deflect entering water downwardly into a drain or gullet chamber. The air intake system typically further includes a mechanism for gravity-mediated draining of water from the air flow conduit to prevent entering water from being drawn into the air compressor or other component.

Abstract: Novel 4-1BB ligand (4-1BB-L) polypeptides and a human cell surface receptor designated 4-1BB that binds 4-1BB-L are provided. Isolated 4-1BB-L-encoding and human 4-1BB-encoding DNA sequences, recombinant expression vectors comprising the isolated DNA sequences, and host cells transformed with the recombinant expression vectors are disclosed, along with methods for producing the novel polypeptides by cultivating such transformed host cells. Soluble forms of the 4-1BB-L or 4-1BB polypeptides are derived from the extracellular domains thereof.

Abstract: Tumor necrosis factor receptor proteins, DNAs and expression vectors encoding TNF receptors, and processes for producing TNF receptors as products of recombinant cell culture, are disclosed.

Abstract: Novel 4-1BB ligand (4-1BB-L) polypeptides and a human cell surface receptor designated 4-1BB that binds 4-1BB-L are provided. Isolated 4-1BB-L-encoding and human 4-1BB-encoding DNA sequences, recombinant expression vectors comprising the isolated DNA sequences, and host cells transformed with the recombinant expression vectors are disclosed, along with methods for producing the novel polypeptides by cultivating such transformed host cells. Soluble forms of the 4-1BB-L or 4-1BB polypeptides are derived from the extracellular domains thereof.

Abstract: A method and system for monitoring performance of an internal combustion engine equipped with individually actuated valve control mechanisms, such as a two-step finger-follower rocker-arm assembly, using a strategy that identifies cylinder-to-cylinder variations, is disclosed. The method and system preferably include monitoring engine operating conditions, based upon input from an exhaust gas sensor and engine sensors, and determining an engine operating point. Individual cylinder fueling modifiers are determined, each corresponding to one of the cylinders at the determined engine operating point, based upon input from the exhaust gas sensor. It is determined that the each individually actuated valve control mechanism is operating properly when a difference between the individual cylinder fueling modifier and a predetermined individual cylinder fueling modifier, each said modifier determined for the corresponding cylinder at the determined engine operating point, is less than a predetermined difference.

Abstract: The invention provides a control strategy and a control system to control a gas sensor to a target operating temperature. It relies upon both feedback and model-based feedforward control systems to achieve and then maintain the sensor at the target operating temperature. The mechanization includes a gas sensor with a heating element in a feedstream. The control strategy employs a control system for the heating element that is based upon the target operating temperature, the temperature of the heating element, and an effect of the feedstream and mounting structure on the temperature of the sensor. The control strategy enables the control system to optimize the heating of a sensor during warm-up and steady state operations.

Abstract: An oxygen sensor heater control determines heater activation based on an open-loop control parameter and a correction factor that compensates for part-to-part variability. Following a cold start where the heater temperature can be reliably estimated, the engine controller predicts the resistance of the heating element and heater circuit at the desired operating temperature of the sensor, and computes the correction factor for heater activation based on the predicted resistance values and nominal resistance values. At least one predicted value is stored in non-volatile memory, and used to compute the correction factor following a warm or hot start of the engine.

Abstract: A digital camera captures high quality long exposure images by capturing and summing several images of the same scene. The effective ISO of the camera is reduced by scaling the summed image, thus reducing image noise and improving long exposure quality.

Abstract: A nozzle design and method for designing such a nozzle being adapted for three phase slurry flow from the choke into the flash tank during pressure let-down in autoclave mining operations that uses a selection of the expansion ratio in the choke to select a nozzle dispersion angle to select a spread of the flow over the slurry pool in the flash tank, to generally maximize the use of the pool for energy dissipation without causing undo wear and tear on the tank walls.

Abstract: Tumor necrosis factor receptor DNAs and expression vectors encoding TNF receptors, and processes for producing TNF receptors as products of recombinant cell culture, are disclosed.

Abstract: N-(Pyridinyl)-1H-indol-1-amines of formula I provide a unique combination of blocking properties for both the potassium and sodium channels. These compounds are useful for the treatment of Demyelinating Diseases and Conditions such as Multiple Sclerosis, Spinal Cord Injury, Traumatic Brain Injury and Stroke. The compounds are also useful for Stroke Rehabilitation, the treatment of Bladder Irritation and Dysfunction, and the treatment of Neuropathic Pain and Chemokine-Induced Pain.

Abstract: In a digital image matching method, reference calibration data associated with one or more reference digital image files is ascertained. The reference digital image files each have reference calibration data that is hidden metadata and can also have public metadata that is readable by a different procedure. A test digital image file is obtained and a data structure capable of holding the reference calibration data is detected in the test digital image file. The presence of second calibration data within the data structure of the test digital image file is determined. If the reference and second calibration data match, then the test digital image file and reference digital image files are from the same digital acquisition device.