Abstract: An apparatus for simultaneously extracting one or more analytes from a plurality of samples, the apparatus comprising a housing having an upper platform having a plurality of pins, each pin suitable for BioSPME, and a lower platform having a plurality of wells for sample solutions, such when the upper platform is fitted over the lower platform, the pins of the upper platform fit into the wells of the lower platform such that the pins are immersed into the samples, allowing for simultaneous extraction of analytes in each sample. The apparatus can be coupled with other instruments to enable measurement of total and free analytes in each sample. Also provided are methods of simultaneous extraction of analytes in a plurality of samples using the apparatus described herein.