Abstract: Methods are provided for measuring the activity of TMEM sites in a tumor comprising measuring a transient increase in permeability of blood vessels at TMEM sites that allows tumor cells to enter the blood vessels, wherein permeability is measured using a modality selected from the group consisting of MRI, PET, CT, and SPECT, and wherein a transient increase in permeability indicates that a TMEM site is active. The method can include, for example, obtaining a MenaINV score assessed by fine needle aspiration in the same tissue. The present invention can be used as both a prognostic for dissemination and a predictive end point for identification and validation of dissemination inhibitors/anti-metastasis drugs.

Abstract: A method of operating a hydrostatically compensated compressed air energy storage system comprising to inhibit champagne effect conditions, can include charging the system; changing the system into a storage mode; and before a dissolved gas concentration in a layer of compensation liquid exceeds a champagne effect saturation threshold, operating the system in a dilution cycle mode that can include i) discharging at least a portion of the compressed air from the accumulator thereby drawing additional compensation liquid having a lower dissolved gas concentration than the liquid in the layer of compensation liquid into the accumulator, and ii) re-charging the system by conveying additional compressed air into the layer of compressed air in the accumulator thereby displacing a corresponding amount of compensation liquid from the layer of compensation liquid into a compensation liquid flow path and returning the system to the storage mode.

Abstract: In one example, a portion of a shed includes a blow molded plastic door, a blow molded plastic wall panel, and a double jointed hinge configured to connect the blow molded plastic door and the blow molded plastic wall panel to each other. The double jointed hinge includes a first hinge element that connectible to the blow molded plastic wall panel, a second hinge element that is connectible to the blow molded plastic door, and a first link and a second link, the first link and the second link configured to connect the first hinge element to the second hinge element, and when the shed portion is in an assembled state, and the blow molded plastic door is fully closed, the first link and the second link are received within a recess cooperatively defined by the blow molded plastic wall panel and the blow molded plastic door.

Abstract: In one example, a double jointed hinge connects a blow molded plastic door and a blow molded plastic wall panel. The double jointed hinge includes a first hinge element that defines a first axis of rotation, and the first hinge element is connected to the blow molded plastic wall panel, a second hinge element that defines a second axis of rotation and is connected to the blow molded plastic door, and the second hinge element is spaced apart from the first hinge element, and a first link and a second link, the first link and the second link connecting the first hinge element to the second hinge element.

Abstract: Methods are provided for measuring the activity of TMEM sites in a tumor comprising measuring a transient increase in permeability of blood vessels at TMEM sites that allows tumor cells to enter the blood vessels, wherein permeability is measured using a modality selected from the group consisting of MRI, PET, CT, and SPECT, and wherein a transient increase in permeability indicates that a TMEM site is active. The method can include, for example, obtaining a MenaINV score assessed by fine needle aspiration in the same tissue. The present invention can be used as both a prognostic for dissemination and a predictive end point for identification and validation of dissemination inhibitors/anti-metastasis drugs.

Abstract: A method of reducing a cerebral tissue pathology associated with a sickle cell disease therapy in a subject is provided using (i) a synthetic high-oxygen affinity hemoglobin-containing molecule or (ii) synthetically treated high-oxygen affinity blood.

Abstract: In one example, a double jointed hinge connects a blow molded plastic door and a blow molded plastic wall panel. The double jointed hinge includes a first hinge element that defines a first axis of rotation, and the first hinge element is connected to the blow molded plastic wall panel, a second hinge element that defines a second axis of rotation and is connected to the blow molded plastic door, and the second hinge element is spaced apart from the first hinge element, and a first link and a second link, the first link and the second link connecting the first hinge element to the second hinge element.

Abstract: A method of reducing a cerebral tissue pathology associated with a sickle cell disease therapy in a subject is provided using (i) a synthetic high-oxygen affinity hemoglobin-containing molecule or (ii) synthetically treated high-oxygen affinity blood.

Abstract: Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. From the resulting two-phase mixture (i.e., a paraffin phase and an aqueous phase), the aqueous phase is collected for purification of nucleic acid. Protease(s) can be used at one or more points in the separation process to facilitate tissue cell lysis and/or degrade proteins that could degrade nucleic acid or interfere with subsequent genetic manipulation or analysis.

Abstract: An apparatus and method for providing RF shielding for performing nuclear magnetic resonance (“NMR”) procedures, comprising a radio-opaque holder in combination with radio-opaque magnet components to form an RF shield around a patient undergoing an NMR procedure. In embodiments, a radio-opaque holder having a radio-opaque bottom portion and a radio-opaque canopy is adjoined to an NMR magnet having a radio-opaque cryostat and a radio-opaque service end cap to form an RF shield. A patient is placed on a patient support unit located in the holder bottom portion. The patient support unit, including the patient, is then inserted into the cavity of the NMR magnet and a canopy is placed on top of the bottom portion of the holder. An RF shield is thus created comprising the canopy, the bottom portion, the cryostat of the magnet, and an end cap on the service end of the magnet.

Abstract: Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. From the resulting two-phase mixture (i.e., a paraffin phase and an aqueous phase), the aqueous phase is collected for purification of nucleic acid. Protease(s) can be used at one or more points in the separation process to facilitate tissue cell lysis and/or degrade proteins that could degrade nucleic acid or interfere with subsequent genetic manipulation or analysis.

Abstract: An apparatus and method for providing RF shielding for performing nuclear magnetic resonance (“NMR”) procedures, comprising a radio-opaque holder in combination with radio-opaque magnet components to form an RF shield around a patient undergoing an NMR procedure. In embodiments, a radio-opaque holder having a radio-opaque bottom portion and a radio-opaque canopy is adjoined to an NMR magnet having a radio-opaque cryostat and a radio-opaque service end cap to form an RF shield. A patient is placed on a patient support unit located in the holder bottom portion. The patient support unit, including the patient, is then inserted into the cavity of the NMR magnet and a canopy is placed on top of the bottom portion of the holder. An RF shield is thus created comprising the canopy, the bottom portion, the cryostat of the magnet, and an end cap on the service end of the magnet.