Abstract: The invention encompasses compositions and methods relating to viral polymerases having one or more substitutions of different amino acids at conserved regions of the polymerase yields enzymes with varying rates and fidelity of replication. A universally applicable, polymerase-mechanism-based strategy for production of attenuated viruses and anti-viral vaccines is disclosed. Attenuated viruses serve as vaccines and were shown to provide protection against poliovirus challenge in vivo.

Abstract: A multicore network security system includes scheduler modules, one or more security modules and post-processing modules. Each security module may be a processing core or itself a network security system. A scheduler module routes input data to the security modules, which perform network security functions, then routes processed data to one or more post-processing modules. The post-processing modules post-process this processed data and route it back to scheduler modules. If further processing is required, the processed data is routed to the security modules; otherwise the processed data is output from the scheduler modules. Each processing core may operate independently from other processing cores, enabling parallel and simultaneous execution of network security functions.

Abstract: The inventors have discovered that an ATPase-deficient dominant-negative mutant NS3 protein of hepatitis C virus inhibits activity of the wild-type NS3 protein and inhibits replication of hepatitis C virus (HCV). The solved crystal structure of a multi-enzyme NS3 complex on a DNA substrate is also provided. The inventors have tested a peptide matching the sequence of a portion of NS3 that interacts with another NS3 molecule for inhibiting HCV replication. The peptide inhibits HCV replication. Accordingly, the invention provides a method of inhibiting HCV replication in cells infected with HCV involving transforming the cells with a vector expressing a dominant-negative mutant NS3 gene. The invention also provides a method of inhibiting HCV replication in cells infected with HCV involving administering to the cells a dominant-negative mutant NS3 protein. The invention also provides peptides and agents that inhibit HCV replication and methods of identifying agents that inhibit HCV replication.

Abstract: A classifier of electronic messages includes one or more pre-filters and a filter. Messages classified as spam or legitimate by one or more of the pre-filters bypass the filter. Messages classified as suspicious are further classified by the filter as either spam or legitimate. Messages classified as spam are routed to a spam quarantine storage area. Messages classified as legitimate are routed to a spam delivery area.

Abstract: Disclosed is a pharmaceutical composition comprising a ribonucleoside analogue in accordance with general formula (I) or (II) as herein defined in admixture with a physiologically acceptable excipient diluent or carrier.