Abstract: A roll-up door assembly including a flexible curtain made of synthetic rubber or fabric, a curtain winding mechanism and two guide members which extend vertically on opposite sides of the doorway. Side edge sections of the curtain are movable in respective guide members. Each guide has inner and outer wall sections with each wall section having an inwardly projecting, longitudinal rib. The two ribs of each guide form an elongate slot that receives a side edge section of the curtain. Spaced-apart pairs of curtain lock members are mounted on and distributed along each side edge section of the curtain. The lock members of each pair are positioned opposite one another on front and rear surfaces of the curtain respectively. The combined thickness of each pair of lock members and the curtain exceeds the width of the elongate slot.