Abstract: Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, a first amplification primer includes one or more deoxyuridine residues, wherein at least one of the one or more deoxyuridine residues is at position ?1, ?2, ?3, ?4 or ?5 of the promoter region for the single-subunit, DNA-dependent RNA polymerase. The deoxyuridines are excised to provide an amplified functional template DNA which is then used to synthesize RNA which has reduced immunogenic double stranded RNA compared to controls.

Abstract: A multi-tool positioning and manufacturing system moves a workpiece among many tools, such as grinding wheels, each of which performs a manufacturing operation on the workpiece. The system moves the workpiece automatically, quickly, repeatably, and precisely among the tools, without any manual intervention. It can perform the same manufacturing operations as many separate tools, such as grinding the inner diameter, outer diameter, and rib of a tapered roller bearing cone. Because a machinist does not have to move the workpiece between machines for different operations, the total manufacturing process can be faster and higher yield than with separate tools. The system can also move a single dresser among the tools for dressing and truing, further increasing manufacturing efficiency by eliminating the need for separate dressers for separate tools.

Abstract: Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.

Abstract: A progressive sealing system for a reciprocating machine includes a pressure breaker having a first seal, and one or more additional seals between the pressure breaker and an end plate. One or more seal housings hold the seals between the first seal and the end plate. The end plate couples the progressive sealing system to a housing of a cylinder of the reciprocating machine such that a surface of the first seal faces a chamber of the cylinder. A housing for the second one of the seals includes a bore having an inner surface. The inner surface of the bore of the second seal housing and an outer surface of a rod of the reciprocating machine define an annular space when the rod is received in the bore. The packing case includes an expansion chamber adjoining the annular space.

Abstract: A seat assembly includes a fabric component with a seat surface and with an interior segment directly attached to the fabric component. The interior segment extends from an exterior segment that has a greater flexural resistance than the interior segment.

Abstract: A valve for a breathing apparatus system includes a housing extending axially from a first end to a second end, with the first end fluidly coupled to the mask and the second end configured to fluidly couple with a source of breathable air. An air flow path extends through the housing between the first end and the second end.

Abstract: The present disclosure provides a rotational spring dampener that has a compression limiter, a first disk, and a second disk. The first disk is disposed at a first end of the compression limiter and the second disk is disposed at a second end of the compression limiter, where the second end is opposite the first end. The rotational spring dampener also has a tensile member. The tensile member is connected to the first disk and the second disk. The tensile member is composed of a block copolymer.

Abstract: A thermal management system for a vehicle includes a heat exchange unit coupled with a compartment of the vehicle via an interface in the compartment. A container is moveable between a first position and a second position. The container is configured to be in fluid communication with the heat exchange unit via the interface in the first position.

Abstract: A method of monitoring degradation of seals of a packing case includes receiving signals from a first temperature sensor installed in a first location in a housing of the packing case; receiving signals from a second temperature sensor installed in a second location in the housing of the packing case; computing, from the signals, a temperature differential between the first temperature sensor and the second temperature sensor at two or more times; and determining, at least in part from the temperature differential at the two or more times, a degradation indicator for at least one of the seals of the packing case.

Abstract: A method of monitoring degradation of seals of a packing case includes receiving signals from a first temperature sensor installed in a first location in a housing of the packing case; receiving signals from a second temperature sensor installed in a second location in the housing of the packing case; computing, from the signals, a temperature differential between the first temperature sensor and the second temperature sensor at two or more times; and determining, at least in part from the temperature differential at the two or more times, a degradation indicator for at least one of the seals of the packing case.

Abstract: A thermal management system for a vehicle includes a heat exchange unit coupled with a compartment of the vehicle via an interface in the compartment. A container is moveable between a first position and a second position. The container is configured to be in fluid communication with the heat exchange unit via the interface in the first position.

Abstract: The present disclosure discloses an apparatus to move a watercraft from one location to another. The apparatus may include a first tire and a second tire, and a first leg and a second leg. Each leg may include a proximal end and a distal end. The first and second legs may be attached to the first and second tires via respective distal ends. The apparatus may further include a first bracket and a second bracket configured to receive the proximal ends of respective first and second legs. The apparatus further includes a first indexing plate and a second indexing plate which are attached to the first bracket and the second bracket. Further, the apparatus includes first and second pull assemblies configured to engage or disengage with the respective first indexing plate and the second indexing plate to move the apparatus between a stow position and transport position.

Abstract: A method for making a seat includes the step of overmolding a carrier onto a fabric seat surface. The fabric surface is formed from a woven fabric material having heating element fibers. The heating element fibers are placed in contact with a conductor. The carrier and the conductor are then positioned into a frame.

Abstract: A load bearing surface includes a woven fabric surface and a carrier (18) overmolded onto the fabric surface. Portions of the woven fabric are bonded to the carriers (18) o as to remain fixed relative to the carrier during normal loading conditions and during abnormally high loading conditions. Other portions (14) of the woven fabric are bonded to the carrier so as to remain fixed relative to the carrier during normal loading conditions and to move or slip within the carrier, or to rupture, during abnormally high loading conditions.

Abstract: A seat assembly includes a fabric component with a seat surface and with an interior segment directly attached to the fabric component. The interior segment extends from an exterior segment that has a greater flexural resistance than the interior segment.

Abstract: Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.

Abstract: The present disclosure provides a rotational spring dampener including a core having an opening therethrough, a first solid component having a first set of bosses, a second solid component having a second set of bosses, and a tensile member positioned between the first solid component and the second solid component. The tensile member is composed of a solid silicon polymer.

Abstract: A system and a method include a fixture template configured to be secured to a component. The fixture template includes a plurality of insert holes. A plurality of guide inserts have channels. Each of the channels has an orientation. The orientation of at least two of the plurality of guide inserts differs. Each of the plurality of guide inserts is removably secured within a respective one of the plurality of inserts holes to position and orient the channels in relation to a pattern of openings to be formed in relation to the component. Each of the channels is configured to receive an operative member of a tool that is configured to form the pattern of openings in relation to the component.

Abstract: According to an embodiment, three-dimensional (3D) scan data representative of an in-service structure that is captured by a scanning device is accessed by a processor circuit. Based on the 3D scan data, in-service computer-aided design (CAD) data representative of an in-service surface corresponding to the in-service structure is generated by the processor circuit. Based on the in-service CAD data representative of the in-service surface, at least one of a serviceability level or remaining life estimate of the in-service structure is determined.