Patents by Inventor Cristina Hartshorn
Cristina Hartshorn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220090216Abstract: Provided herein are reagents for improving PCR accuracy.Type: ApplicationFiled: September 28, 2021Publication date: March 24, 2022Inventors: John RICE, Lawrence WANGH, Arthur H. REIS, JR., Kenneth PIERCE, Cristina HARTSHORN, J. Aquiles SANCHEZ, Stephen VAN HOOSER, Skye FISHBEIN
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Patent number: 11162146Abstract: Provided herein are reagents for improving PCR accuracy.Type: GrantFiled: April 1, 2019Date of Patent: November 2, 2021Assignee: Brandeis UniversityInventors: John Rice, Lawrence Wangh, Arthur H. Reis, Jr., Kenneth Pierce, Cristina Hartshorn, J. Aquiles Sanchez, Stephen Van Hooser, Skye Fishbein
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Publication number: 20190292610Abstract: Provided herein are reagents for improving PCR accuracy.Type: ApplicationFiled: April 1, 2019Publication date: September 26, 2019Inventors: John RICE, Lawrence Wangh, Arthur H. Reis, JR., Kenneth Pierce, Cristina Hartshorn, J. Aquiles Sanchez, Stephen Van Hooser, Skye Fishbein
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Patent number: 10301688Abstract: Provided herein are reagents for improving PCR accuracy.Type: GrantFiled: January 23, 2014Date of Patent: May 28, 2019Assignee: BRANDEIS UNIVERSITYInventors: John Rice, Lawrence Wangh, Arthur H. Reis, Jr., Kenneth Pierce, Cristina Hartshorn, J. Acquiles Sanchez, Stephen Van Hooser, Skye Fishbein
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Patent number: 9745624Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.Type: GrantFiled: August 1, 2012Date of Patent: August 29, 2017Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, John E. Rice, J. Aquiles Sanchez, Kenneth E. Pierce, Jesse Salk, Arthur H. Reis, Cristina Hartshorn
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Patent number: 9476092Abstract: A non-symmetric polymerize chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.Type: GrantFiled: January 4, 2013Date of Patent: October 25, 2016Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J. Aquiles Sanchez
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Patent number: 9279121Abstract: Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents.Type: GrantFiled: November 10, 2008Date of Patent: March 8, 2016Inventors: Lawrence J Wangh, Cristina Hartshorn
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Publication number: 20140206564Abstract: Provided herein are reagents for improving PCR accuracy.Type: ApplicationFiled: January 23, 2014Publication date: July 24, 2014Applicant: Brandeis UniversityInventors: John Rice, Lawrence Wangh, Arthur H. Reis, JR., Kenneth Pierce, Cristina Hartshorn, J. Aquiles Sanchez, Stephen Van Hooser, Skye Fishbein
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Publication number: 20130210656Abstract: A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.Type: ApplicationFiled: January 4, 2013Publication date: August 15, 2013Applicant: Brandeis UniversityInventors: Lawrence J. Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J. Aquiles Sanchez
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Publication number: 20130095479Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.Type: ApplicationFiled: August 1, 2012Publication date: April 18, 2013Applicant: BRANDEIS UNIVERSITYInventors: Lawrence J. Wangh, John Rice, J. Aquilles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn
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Patent number: 8367325Abstract: A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.Type: GrantFiled: February 2, 2007Date of Patent: February 5, 2013Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J. Aquiles Sanchez
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Publication number: 20120198576Abstract: Embodiments of the invention are based upon the discovery that exposure of cleavage-stage embryos to a stress inducer, e.g. heat shock or chemical, renders the exposed embryos more sensitive to a secondary treatment with a stress inducer, e.g. heat shock or chemical inducer. Accordingly, the present invention is directed to methods for making embryos, embryonic cells arising from them, and animals and plants that are sensitized to stress, e.g. physiologic or chemical stressors. Methods of screening for inducers and inhibitors of stress using, as test model systems, embryonic cells, embryos, animals, and plants that are sensitized to stress are also disclosed.Type: ApplicationFiled: July 1, 2010Publication date: August 2, 2012Applicant: BRANDEIS UNIVERSITYInventors: Lawrence J. Wangh, Cristina Hartshorn, Yanwei Jia
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Publication number: 20120040352Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.Type: ApplicationFiled: November 10, 2008Publication date: February 16, 2012Inventors: Lawrence J. Wangh, John Rice, J. Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn
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Publication number: 20110311971Abstract: An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described.Type: ApplicationFiled: May 9, 2011Publication date: December 22, 2011Applicant: BRANDEIS UNIVERSITYInventors: Cristina Hartshorn, Kenneth E. Pierce, Arthur Henry Reis, JR., John E. Rice, J. Aquiles Sanchez, Lawrence J. Wangh
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Patent number: 7972786Abstract: An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described.Type: GrantFiled: July 6, 2007Date of Patent: July 5, 2011Assignee: Brandeis UniversityInventors: Cristina Hartshorn, Kenneth E. Pierce, Arthur Henry Reis, Jr., John E. Rice, J. Aquiles Sanchez, Lawrence J. Wangh
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Patent number: 7632642Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.Type: GrantFiled: October 17, 2005Date of Patent: December 15, 2009Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, John Rice, J. Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn
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Publication number: 20090226973Abstract: An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.Type: ApplicationFiled: March 20, 2009Publication date: September 10, 2009Inventors: Lawrence J. Wangh, John Rice, J. Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn
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Publication number: 20090111170Abstract: Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents.Type: ApplicationFiled: November 10, 2008Publication date: April 30, 2009Inventors: Lawrence J. Wangh, Cristina Hartshorn
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Patent number: 7517977Abstract: An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.Type: GrantFiled: October 17, 2005Date of Patent: April 14, 2009Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, John Rice, J. Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn
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Patent number: 7465562Abstract: Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents.Type: GrantFiled: May 18, 2004Date of Patent: December 16, 2008Assignee: Brandeis UniversityInventors: Lawrence J. Wangh, Cristina Hartshorn