Patents by Inventor Cristina PAROLA

Cristina PAROLA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • MAMMALIAN CELL LINE FOR PROTEIN PRODUCTION AND LIBRARY GENERATION
    Publication number: 20230374491
    Abstract: According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.
    Type: Application
    Filed: June 5, 2023
    Publication date: November 23, 2023
    Applicant: ETH ZURICH
    Inventors: Sai REDDY, William KELTON, Cristina PAROLA, Derek MASON, Mark POGSON
  • Mammalian cell line for protein production and library generation
    Patent number: 11802281
    Abstract: According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.
    Type: Grant
    Filed: March 17, 2017
    Date of Patent: October 31, 2023
    Inventors: Sai Reddy, William Kelton, Cristina Parola, Derek Mason, Mark Pogson
  • MAMMALIAN CELL LINE FOR PROTEIN PRODUCTION AND LIBRARY GENERATION
    Publication number: 20200325468
    Abstract: According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.
    Type: Application
    Filed: March 17, 2017
    Publication date: October 15, 2020
    Applicant: ETH ZURICH
    Inventors: Sai REDDY, William KELTON, Cristina PAROLA, Derek MASON, Mark POGSON