Abstract: This disclosure relates to a rapid molecular imaging strategy that utilizes Raman spectroscopy to interrogate the molecular expression of samples, for example, histology samples. This disclosure also relates to surface-enhanced Raman spectroscopy nanoparticles (SERS-NP) as contrast agents that exhibit unsurpassed multiplexing capabilities to offer exceptional specificity. This disclosure also relates to a SERS-NP comprising a Raman active metallic core, a Raman active layer, and a shell that may offer ultra-high diagnostic sensitivity (e.g., femtomolar level). This disclosure also relates to chemically modifying these new contrast agents to target a plurality of biomarkers within the sample. This disclosure also relates to rapid assessment of the molecular expression profile of the entire sample, for example, a whole tissue section, in a single image.

Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to Raman imaging devices (e.g., Raman endoscope probes), methods of using Raman agents and Raman imaging devices to image or detect a signal, and the like.

Abstract: The present disclosure describes a Raman topography system, which includes a container with at least one Raman probe positioned within. Also described are methods of imaging which include the Raman topography system.

Abstract: The present disclosure provides for a Raman probes and methods of imaging including a Raman probe. The Raman probes include a Raman detection system configured to illuminate an area of a subject or a sample with a light source and to receive Raman scattered light energy from the area. The Raman probe can include a proximity sensor system and a fluorescent imaging system. A method of imaging introduces a Raman probe to a subject. Fluorescent light is detected from an area of the subject, which guides the Raman probe to the area. The Raman probe is positioned at a target distance from the area using the proximity sensor system, and by exposing the area to a light beam from the Raman detection system. The light beam, Raman scattered light energy, is scattered by a Raman agent associated with the area. Raman scattered light is detected using the Raman imaging device.

Abstract: The present disclosure provides for a Raman probes and methods of imaging including a Raman probe. The Raman probes include a Raman detection system configured to illuminate an area of a subject or a sample with a light source and to receive Raman scattered light energy from the area. The Raman probe can include a proximity sensor system and a fluorescent imaging system. A method of imaging introduces a Raman probe to a subject. Fluorescent light is detected from an area of the subject, which guides the Raman probe to the area. The Raman probe is positioned at a target distance from the area using the proximity sensor system, and by exposing the area to a light beam from the Raman detection system. The light beam, Raman scattered light energy, is scattered by a Raman agent associated with the area. Raman scattered light is detected using the Raman imaging device.

Abstract: The present disclosure describes a Raman topography system, which includes a container with at least one Raman probe positioned within. Also described are methods of imaging which include the Raman topography system.

Abstract: Embodiments of the present disclosure provide for probes, methods of using the probe, methods of making the probe, method of imaging a condition (e.g., pre-cancerous tissue, cancer, or a tumor), methods of planning resection of a brain tumor, methods of imaging a brain tumor, and the like.

Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to Raman imaging devices (e.g., Raman endoscope probes) or systems, methods of using Raman agents, Raman imaging devices, and/or systems to image or detect a signal, and the like.

Abstract: Embodiments of the present disclosure provide for probes, methods of using the probe, methods of making the probe, method of imaging a condition (e.g., pre-cancerous tissue, cancer, or a tumor), methods of planning resection of a brain tumor, methods of imaging a brain tumor, and the like.

Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to Raman imaging devices (e.g., Raman endoscope probes), methods of using Raman agents and Raman imaging devices to image or detect a signal, and the like.