Abstract: Microbial growth of at least one microorganism may be decreased, prevented, and/or inhibited in a fluid by providing ultrasonic waves to the fluid. In an optional non-limiting embodiment, an amount of at least one gas may be provided to the fluid for further decreasing the microbial population, preventing microbial growth, and/or inhibiting microbial growth. In another non-limiting embodiment, the fluid may be a downhole fluid, such as but not limited to, drilling fluids, completion fluids, production fluids, injection fluids, stimulation fluids, refinery fluids, servicing fluids, and combinations thereof. Alternatively, the fluid or downhole fluid may have a temperature ranging from about 25 C to about 100 C.

Abstract: At least one nucleic acid from a sulfate-reducing bacteria (SRB) may be extracted from an oilfield fluid and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may be or include a sequence including, but not necessarily limited to, SEQ ID NO: 20, SEQ ID NO: 21, and mixtures thereof. The amplification product may be hybridized with a probe specific for a fragment of an alpha subunit of an APS gene, and a presence of hybridization and a degree of hybridization may be detected.

Abstract: Biofouling may be prevented or at least mitigated by employing a cinnamaldehyde additive to augment the affect of the conventional biocide. Exemplary cinnamaldehyde additives include, but are not limited to, cinnamaldehyde, cinnamic acid and cinnamyl alcohol. A cinnamaldehyde additive by itself, in some embodiments, may also inhibit biofouling.

Abstract: At least one nucleic acid from a sulfate-reducing bacteria (SRB) may be extracted from an oilfield fluid and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may be or include a sequence including, but not necessarily limited to, SEQ ID NO:1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and mixtures thereof. The amplification product may be hybridized with a probe specific for a fragment of an alpha subunit of an APS gene, and a presence of hybridization and a degree of hybridization may be detected.

Abstract: At least one nucleic acid from a sulphate-reducing bacteria may be extracted from an oilfield fluid and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may be or include a sequence essentially identical to SEQ ID NO:1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, and mixtures thereof. The amplification product may be hybridized with a probe specific for a fragment of an alpha subunit of an APS gene, and a presence of hybridization and a degree of hybridization may be detected.

Abstract: Microbial growth of at least one microorganism may be decreased, prevented, and/or inhibited in a fluid by providing ultrasonic waves to the fluid. In an optional non-limiting embodiment, an amount of at least one gas may be provided to the fluid for further decreasing the microbial population, preventing microbial growth, and/or inhibiting microbial growth. In another non-limiting embodiment, the fluid may be a downhole fluid, such as but not limited to, drilling fluids, completion fluids, production fluids, injection fluids, stimulation fluids, refinery fluids, servicing fluids, and combinations thereof. Alternatively, the fluid or downhole fluid may have a temperature ranging from about 25 C to about 100 C.

Abstract: A kit for optional use with a PCR method of amplification may include at least one reaction well, and an internal amplification control for a PCR amplification of an APS reductase gene having a sequence complementary to at least one sequence essentially identical to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, and mixtures thereof. The kit may be used with a PCR method of amplifying at least one sulfur-reducing bacteria extracted from an oilfield fluid.

Abstract: Biofouling may be prevented or at least mitigated by employing a cinnamaldehyde additive to augment the affect of the conventional biocide. Exemplary cinnamaldehyde additives include, but are not limited to, cinnamaldehyde, cinnamic acid and cinnamyl alcohol. A cinnamaldehyde additive by itself, in some embodiments, may also inhibit biofouling.

Abstract: DNA/RNA monitoring of microbes at an oil field to determine the presence and activity of harmful microbes is accomplished with a portable qPCR (quantitative polymerase chain reaction) machine. This permits the monitoring to occur on-site in the field and reduces the variability that may occur from the transportation of samples.