Abstract: A disposable, fabrication-free, high-volume electroporation device may process cell samples of large volume without compromising transformation efficiency and cell viability, while precipitously reducing the entire processing time and effort. An embodiment includes at least two hollow, tubular conductive elements and an insulating structure, defining a channel, that fluidically couples the at least two conductive elements to define an electroporation flow path in the channel for flow-through electroporation. The high-volume electroporation device can be an alternative to cuvettes for typical volume electroporation, but can also be an indispensable tool to process large volume samples for applications, such as creation of modified sample libraries.

Abstract: An electroporation assembly for interfacing with a liquid handling system includes at least two electrically conductive layer components and a channel layer component. The channel layer component is configured to be removably disposed between the electrically conductive layer components. The electrically conductive layer components and the channel layer component define a plurality of parallel electroporation flow paths in an assembled state. An electroporation system includes an electroporation assembly and a cell collection unit. The electroporation assembly is configured to direct a flow of a cell sample to the cell collection unit.

Abstract: An electroporation device with a volume of varying cross sectional area that as a fast assay device for determining the optimal conditions for plasma membrane electroporation.

Abstract: An electroporation device with a volume of varying cross-sectional area that be used as a fast assay device for determining the optimal conditions for plasma membrane electroporation.

Abstract: The invention discloses general apparatus and methods for electrochemical energy conversion and storage via a membraneless laminar flow battery. In a preferred embodiment, the battery includes a flow-through porous anode for receiving a fuel and a porous electrolyte channel for transporting an electrolyte adjacent to the porous anode; a flow-through porous cathode is provided for transporting an oxidant; and a porous dispersion blocker is disposed between the electrolyte channel and the porous cathode, which inhibits convective mixing while allowing molecular diffusion and mean flow. Pore structure properties are selected for tuning convective dispersion, conductivity or other macroscopic properties. Specific materials, reactants, fabrication methods, and operation methods are disclosed to achieve stable charge/discharge cycles and to optimize power density and energy density.

Abstract: Methods and apparatuses for cell electroporation are provided. An apparatus includes a fluid transport structure, such as a pipette, that includes an electroporation structure defining a flow path having a constriction. The fluid transport structure also includes at least two conductive elements configured to produce an electric field in the flow path. The conductive elements are in operative arrangement with each other and are configured to expose cells contained within a cell suspension flowing through the fluid transport structure to an electric field that is sufficient to electroporate at least a subset of the cells in the flow path.

Abstract: An electroporation device with a volume of varying cross sectional area that as a fast assay device for determining the optimal conditions for plasma membrane electroporation.

Abstract: A method of altering a property of a surface includes suspending a plurality of low surface energy particles in a solvent, agglomerating the suspension of particles, and subjecting the suspension of particle agglomerates to electrophoretic deposition onto a substrate for a predetermined time. The altered surface may be superhydrophilic or superhydrophobic.

Abstract: A method of altering a property of a surface includes suspending a plurality of low surface energy particles in a solvent, agglomerating the suspension of particles, and subjecting the suspension of particle agglomerates to electrophoretic deposition onto a substrate for a predetermined time. The altered surface may be superhydrophilic or superhydrophobic.

Abstract: The invention discloses general apparatus and methods for electrochemical energy conversion and storage via a membraneless laminar flow battery. In a preferred embodiment, the battery includes a flow-through porous anode for receiving a fuel and a porous electrolyte channel for transporting an electrolyte adjacent to the porous anode; a flow-through porous cathode is provided for transporting an oxidant; and a porous dispersion blocker is disposed between the electrolyte channel and the porous cathode, which inhibits convective mixing while allowing molecular diffusion and mean flow. Pore structure properties are selected for tuning convective dispersion, conductivity or other macroscopic properties. Specific materials, reactants, fabrication methods, and operation methods are disclosed to achieve stable charge/discharge cycles and to optimize power density and energy density.

Abstract: A method of altering a property of a surface includes suspending a plurality of low surface energy particles in a solvent, agglomerating the suspension of particles, and subjecting the suspension of particle agglomerates to electrophoretic deposition onto a substrate for a predetermined time. The altered surface may be superhydrophilic or superhydrophobic.