Abstract: Provided is a robust nasal organoid culture system and fully characterized 2D and 3D in-vitro generated organoids, and methods of using the organoids. The nasal organoids contain a population of differentiated nasal epithelial cells having cellular heterogeneity and molecular markers similar to mature nasal epithelial tissue. Improved methods for generating two-dimensional and three-dimensional nasal organoids are also provided. The methods generally require a two-phase culture system, i.e., expansion and differentiation culture. The provided nasal organoids are suitable for recapitulating nasal tissue complexity for modeling respiratory diseases, studying barrier function and drug delivery, high-throughput drug screening, and assessing the effects of pathogens and environmental toxins on the nasal respiratory epithelium.

Abstract: The disclosure is generally directed to a composition, method and system of respiratory organoids that sustain propagation of respiratory pathogens. Also provided is a method of modeling a biological process in a human lung. Also provided is a method of screening for compounds that affect respiratory pathogens in a respiratory organoid model.

Abstract: Methods for obtaining a population of differentiated alveolar cells, differentiated alveolar cells in the form of alveolar organoids generated by the methods and uses for the differentiated alveolar organoids are provided. The methods include digesting long-term expanding lung organoids (LO) into single cells (i.e., dissociated cells), and suspension culturing the dissociated cells in distal differentiation (DD) cell culture media in a non-adherent plate for an effective amount of time. Alveolar organoids includes a population of AT1 and AT2 cells and in suspension culture exhibit an apical-out polarity and include abundant cytoplasmic lamellar bodies and microvilli. The alveolar organoids can be utilized alone or in combination with 2D and 3D AWO, rebuilt the human respiratory epithelium in culture plates for studying biology and pathology of human respiratory system, including, but not limited to, the study of COVID-19 respiratory diseases.

Abstract: The invention relates to the technical field of medicine, and provides a method of screening and identifying small molecule with antiviral properties. The invention has screened and identified Cannabidiol (CBD), which can be used for prevention of viral infections. The invention also examined and verified that CBD can be used for prevention of COVID-19 disease, by a key signaling pathway of upregulating the expression of the TRIB3 gene. The invention also determined that the optimal dosage of CBD treatment for prevention of COVID-19 disease is 5 ?M-10 ?M.

Abstract: Provided are methods for generating 2D and 3D differentiated airway organoids, 2D and 3D differentiated airway organoids which are generated by the methods and uses for the 2D and 3D differentiated airway organoids.

Abstract: The present invention provides a ternary complex on rare earth earth-amino acid-vitamin used as plant growth regulator. The complex has the general formula: REX-(AA)Y-VI-SZ,in which X=1-2;Y=0-2;Z=0-5;RE is a tervalent ion selected optionally from La, Ce, Pr, Nd and Sm; the primary ligand (AA) is amino acid selected from cystine, tyrosine, glycine, glutamic acid, leucine, proline, lysine, phenylalanine, threonine, valine and so on; the secondary ligand VI is vitamin selected from vitamin B, vitamin C, vitamin D and so on, the solvent S is one or more of tetrahydrofuran, methanol, ethanol, and/or dimethylsulfoxide. The yield of plant with root tuber or stem tuber can be increased by 20-56% and the quality thereof can be improved by 1-2 grades when applying such plant growth regulator on the plant. The advantages of the preparation method thereof lie in lesser processes and shorter reaction period.