Abstract: A liquid yeast one- and two-hybrid high-throughput library screening method and use thereof changes the screening of solid medium to liquid culture solution screening, avoiding a large number of plate coating work, and avoiding the tedious work of identifying single colonies one by one, which not only saves experiment time, but also reduces the cost and workload; at the same time, the present invention also combines high-throughput sequencing technology to improve the analysis throughput of yeast hybridization screening work, and can also improve the automation level of analysis, and can greatly reduce false negatives. More importantly, the present invention can solve the phenomenon of protein self-activation. By changing to liquid screening, known protein interaction information can be successfully obtained, which provides a new opportunity for protein-protein interaction research.

Abstract: Some embodiments of the present disclosure provide a hybrid power coupling mechanism and a vehicle. The hybrid power coupling mechanism includes an engine, a first output shaft, a generator, a driving motor, and a differential mechanism, wherein a rotating shaft of the engine is connected to an input shaft of the generator through the first output shaft, and an input shaft of the driving motor is connected to the differential mechanism in a speed-reducing manner through a planetary gear set. The vehicle includes a power storage battery, a motor controller and the foregoing hybrid power coupling mechanism. The problems of power interruption and high cost in mode switching of a hybrid power coupling mechanism of the existing vehicle are solved. The engine and the generator operate in an extended range mode, and the driving motor is decelerated by the planetary gear set and inputs power to the differential mechanism.

Abstract: Some embodiments of the present disclosure provide a hybrid power coupling mechanism and a vehicle. The hybrid power coupling mechanism includes an engine, a first output shaft, a generator, a driving motor, and a differential mechanism, wherein a rotating shaft of the engine is connected to an input shaft of the generator through the first output shaft, and an input shaft of the driving motor connected to the differential mechanism in a speed-reducing manner through a planetary gear set. The vehicle includes a power storage battery, a motor controller and the foregoing hybrid power coupling mechanism. The problems of power interruption and high cost in mode switching of a hybrid power coupling mechanism of the existing vehicle are solved. The engine and, the generator operate in an extended range mode, and the driving motor is decelerated by the planetary gear set and inputs power to the differential mechanism.