Abstract: A filter assembly for filtering a fluid. The filter assembly at least one filter in a housing, an inlet lid mounted over the filter housing and including an inlet for the inlet of fluid into the housing, and an outlet lid mounted over the filter housing and including an outlet for the outlet of fluid from the housing. The assembly also includes an inlet plunger adapted for sealing flow from the inlet when the inlet plunger is biased to a first sealing position, and an outlet plunger adapted for sealing flow to the outlet when the outlet plunger is biased to a first sealing position.

Abstract: The present invention relates to expression cassettes libraries of expression vectors comprising the same, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of another in the library and cells comprising the same. Methods utilizing either the libraries or cells of this invention, in optimizing gene expression, protein expression, or optimized gene or protein delivery are described.

Abstract: A filter assembly for filtering a fluid. The filter assembly at least one filter in a housing, an inlet lid mounted over the filter housing and including an inlet for the inlet of fluid into the housing, and an outlet lid mounted over the filter housing and including an outlet for the outlet of fluid from the housing. The assembly also includes an inlet plunger adapted for sealing flow from the inlet when the inlet plunger is biased to a first sealing position, and an outlet plunger adapted for sealing flow to the outlet when the outlet plunger is biased to a first sealing position.

Abstract: The present invention relates to expression vectors, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of the wild-type promoter and cells comprising the same. Methods utilizing either the vectors or cells of this invention, in optimizing regulation of gene expression, protein expression, or optimized gene or protein delivery are described.

Abstract: The present invention relates to expression cassettes libraries of expression vectors comprising the same, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of another in the library and cells comprising the same. Methods utilizing either the libraries or cells of this invention, in optimizing gene expression, protein expression, or optimized gene or protein delivery are described.