Abstract: Structures for reducing forward loads in compressors are described. compressor includes inner and outer circumferential support structures positioned concentrically around a central axis, and an aft-most stage including a vane extending radially inward from the outer circumferential support structure. An axial length of the aft-most stage is defined by a spacer arm of the inner circumferential support structure. The vane includes a root, a tip, and a trailing edge extending between the root and the tip. A ratio of a first radial distance between a first point located at an intersection of the tip of the vane and the trailing edge to a radially inner wall of the spacer arm of the inner circumferential support structure to a second radial distance between the first point and a second point located at an intersection of the root of the vane and the trailing edge is between 0.95 and 4.5.

Abstract: The present disclosure relates generally to methods, isolated polypeptides and polynucleotides, expression vectors, and host cells for the production of olivetolic acid and phytocannabinoids. A method of producing olivetolic acid (OVLa) and/or a phytocannabinoid in a heterologous host cell having OVLa-producing or phytocannabinoid-producing capacity comprises transforming the host cell with a nucleotide encoding a variant olivetolic acid cyclase (OAC) protein having at least 6 amino acid mutations relative to the wild type OAC protein, and culturing the transformed host cell to produce OVLa and/or phytocannabinoids therefrom. The variant OAC protein (SEQ ID NO:92) has at least 85% sequence identity with the wild type OAC protein (SEQ ID NO:91). Exemplary variants having improved OVLa or phytocannabinoid production capacity are described.

Abstract: The present disclosure relates generally to methods, isolated polypeptides and polynucleotides, expression vectors, and host cells for the production of cannabidiolic acid (CBDa), cannabigerolic acid (CBCa), and other phytocannabinoids. A method of producing CBDa, CBCa, and/or other phytocannabinoids in a heterologous host cell having CBDa-producing, CBCa-producing or phytocannabinoid-producing capacity comprises transforming the host cell with a nucleotide encoding a variant CBDa synthase protein having a serine insertion between residues P224 and K225 and one or more other amino acid mutation relative to wild type CBDa synthase, and culturing the transformed host cell to produce CBDa, CBCa, and/or other phytocannabinoids therefrom. The variant CBDa synthase protein has at least 85% sequence identity with the wild type CBDa synthase protein sequence OXC52 according to SEQ ID NO:140, with serine insertion (SEQ ID NO:141). Exemplary variants having good phytocannabinoid production capacity are described.

Abstract: The present disclosure relates generally to methods, isolated polypeptides and polynucleotides, expression vectors, and host cells for the production of olivetolic acid and phytocannabinoids. A method of producing olivetolic acid (OVLa) and/or a phytocannabinoid in a heterologous host cell having OVLa-producing or phytocannabinoid-producing capacity comprises transforming the host cell with a nucleotide encoding a variant olivetolic acid cyclase (OAC) protein having at least 6 amino acid mutations relative to the wild type OAC protein, and culturing the transformed host cell to produce OVLa and/or phytocannabinoids therefrom. The variant OAC protein (SEQ ID NO:92) has at least 85% sequence identity with the wild type OAC protein (SEQ ID NO:91). Exemplary variants having improved OVLa or phytocannabinoid production capacity are described.

Abstract: The present disclosure relates generally to methods and cell lines for the production of phytocannabinoids, phytocannabinoid precursors or intermediates, or phytocannabinoid analogue. Methods for transformation of host cells, such as yeast cells, are described. Cells may be transformed, for example, with a polynucleotide encoding a polyketide synthase (PKS) enzyme, a polynucleotide encoding an olivetolic acid cyclase (OAC) enzyme, and/or a polynucleotide encoding a prenyltransferase (PT) enzyme; and optionally a polynucleotide encoding an acyl-CoA synthase (Alk) enzyme; a polynucleotide encoding a fatty acyl CoA activating (CsAAE) enzyme; and/or a polynucleotide encoding a THCa synthase (OXC) enzyme.

Abstract: An engine component assembly with a first substrate having a hot surface in thermal communication with a hot combustion gas flow and a cooling surface, with the cooling surface being different than the hot surface, a second substrate having a first surface in fluid communication with a cooling fluid supply and a second surface, different from the first surface, facing and spaced from the cooling surface to define at least one interior cavity. At least one cavity is provided within the first substrate defining a cavity surface where at least a portion of the cavity surface is directly opposite the second surface. At least one cooling aperture extending through the second substrate from the first surface to the second surface, and defining a streamline along which a cooling fluid passes from the cooling fluid supply to the at least one interior cavity.

Abstract: The present disclosure relates generally to methods, isolated polypeptides and polynucleotides, expression vectors, and host cells for the production of olivetolic acid and phytocannabinoids. A method of producing olivetolic acid (OVLa) and/or a phytocannabinoid in a heterologous host cell having OVLa-producing or phytocannabinoid-producing capacity comprises transforming the host cell with a nucleotide encoding a variant olivetolic acid cyclase (OAC) protein having at least 6 amino acid mutations relative to the wild type OAC protein, and culturing the transformed host cell to produce OVLa and/or phytocannabinoids therefrom. The variant OAC protein (SEQ ID NO:92) has at least 85% sequence identity with the wild type OAC protein (SEQ ID NO:91). Exemplary variants having improved OVLa or phytocannabinoid production capacity are described.

Abstract: An engine component assembly includes a first engine component having a hot surface in thermal communication with a hot combustion gas flow and a cooling surface with at least one cavity. A second engine component is spaced from the cooling surface, and includes at least one cooling aperture. The cooling aperture is arranged such that cooling fluid impinges on the cooling surface at an angle.

Abstract: A flow transfer apparatus for transferring cooling flow from a primary gas flowpath to a turbine rotor. The apparatus includes a first supply plenum communicating with the primary gas flowpath and first inducers, the first inducers configured to accelerate a first fluid flow from the first supply plenum and discharge it toward the rotor with a tangential velocity; a second supply plenum communicating with the primary gas flowpath and second inducers, the second inducers configured to accelerate a second fluid flow from the second supply plenum towards the rotor with a tangential velocity; and a cooling modulation valve operable to selectively permit or block the second fluid flow from the primary gas flowpath to the second supply plenum. The valve includes a flow control structure disposed in the primary gas flowpath and an actuation structure extending to a location radially outside of a casing defining the primary gas flowpath.

Abstract: A centrifugal separator for removing particles from a fluid stream includes an angular velocity increaser configured to increase the angular velocity of a fluid stream, a flow splitter configured to split the fluid stream to form a concentrated-particle stream and a reduced-particle stream, and an exit conduit configured to receive the reduced-particle stream. An inducer assembly for a turbine engine includes an inducer with a flow passage having an inducer inlet and an inducer outlet in fluid communication with a turbine section of the engine, and a particle separator, which includes a particle concentrator that receives a compressed stream from a compressor section of the engine and a flow splitter. A turbine engine includes a cooling air flow circuit which supplies a fluid stream to a turbine section of the engine for cooling, a particle separator located within the cooling air flow circuit, and an inducer forming a portion of the cooling air flow circuit in fluid communication with the particle separator.

Abstract: Gas turbine engine components are provided which utilize an insert to provide cooling air along a cooled surface of an engine component. The insert provides cooling holes or apertures which face the cool side surface of the engine component and direct cooling air onto that cool side surface. The apertures may be formed in arrays and directed at an oblique or a non-orthogonal angle to the surface of the insert and may be at an angle to the surface of the engine component being cooled. An engine component assembly is provided with counterflow impingement cooling, comprising an engine component cooling surface having a cooling fluid flow path on one side and a second component adjacent to the first component. The second component may have a plurality of openings forming an array wherein the openings extend through the second component at a non-orthogonal angle to the surface of the second component.

Abstract: A heat exchanger assembly for a gas turbine engine that includes an outer engine case. The heat exchanger assembly includes at least one cooling channel, the at least one cooling channel is configured to receive a flow of fluid to be cooled. At least one first coolant flow duct that is configured to receive a flow of a first coolant, wherein the at least one cooling channel is disposed between a first inlet and a first outlet. The heat exchanger assembly further include at least one second coolant flow duct that is configured to receive a flow of a second coolant, wherein the at least one cooling channel is disposed between a second inlet and a second outlet.

Abstract: A structure for disrupting the flow of a fluid comprises and a second lateral wall spaced apart from one another, yet joined, by a floor and a ceiling; and, (b) a vorticor pin extending in a direction parallel to an X-axis, the vorticor pin concurrently rising above and extending away from the floor to a height, in a direction parallel to a Y-axis, the vorticor pin comprising: (i) a front surface extending incompletely between the first lateral wall and the second lateral wall, the front surface extending above the floor and having an arcuate portion that is transverse with respect to a Z-axis, which is perpendicular to the X-axis and the Y-axis, and (ii) a rear surface extending between the first lateral wall and the second lateral wall, the rear surface extending between the front surface and the floor, the rear surface having an inclining section that tapers in height, taken parallel to the Y-axis, in a direction parallel to the Z-axis.

Abstract: A flow transfer apparatus for transferring cooling flow from a primary gas flowpath to a turbine rotor. The apparatus includes a first supply plenum communicating with the primary gas flowpath and first inducers, the first inducers configured to accelerate a first fluid flow from the first supply plenum and discharge it toward the rotor with a tangential velocity; a second supply plenum communicating with the primary gas flowpath and second inducers, the second inducers configured to accelerate a second fluid flow from the second supply plenum towards the rotor with a tangential velocity; and a cooling modulation valve operable to selectively permit or block the second fluid flow from the primary gas flowpath to the second supply plenum. The valve includes a flow control structure disposed in the primary gas flowpath and an actuation structure extending to a location radially outside of a casing defining the primary gas flowpath.

Abstract: A shroud assembly for a turbine section of a turbine engine includes a shroud plate in thermal communication with a hot combustion gas flow and a baffle overlying the shroud plate to define a region. One or more shaped cooling features are located along the region such that a cooling fluid flow passing through the region encounters the shaped cooling features to increase the turbulence of the cooling fluid flow.

Abstract: A flow transfer apparatus for transferring cooling flow from a primary gas flowpath to a turbine rotor. The apparatus includes a first supply plenum communicating with the primary gas flowpath and first inducers, the first inducers configured to accelerate a first fluid flow from the first supply plenum and discharge it toward the rotor with a tangential velocity; a second supply plenum communicating with the primary gas flowpath and second inducers, the second inducers configured to accelerate a second fluid flow from the second supply plenum towards the rotor with a tangential velocity; and a cooling modulation valve operable to selectively permit or block the second fluid flow from the primary gas flowpath to the second supply plenum. The valve includes a flow control structure disposed in the primary gas flowpath and an actuation structure extending to a location radially outside of a casing defining the primary gas flowpath.

Abstract: A shroud assembly for a gas turbine engine includes a shroud, hanger, and a hanger support mounted adjacent to a plurality of blades. The hanger can have an interior chamber defining a cooling circuit with a particle separator located within the interior chamber. The particle separator can have an inlet for accepting a flow of cooling fluid, such that a the flow of cooling fluid separates into a major flow and a minor flow carrying particles or particulate matter along the minor flow into a particle collector comprising at least a portion of the particle separator. Particles become constrained to the minor flow and pass into the particle collector, while the major flow is separated into the remaining area of the interior chamber to remove the particles from the flow of cooling fluid passing into the interior chamber.

Abstract: A shroud assembly for a turbine engine includes a shroud having a front side confronting the blades of a turbine and a back side opposite the front side, a hanger configured to couple the shroud to with casing of the turbine, a cooling conduit extending through the hanger to supply a cooling fluid stream to the back side of the shroud, and a particle separator having a through passage forming part of the cooling conduit and a scavenge conduit branching from the through passage.

Abstract: A heat exchanger assembly for a gas turbine engine that includes an outer engine case. The heat exchanger assembly includes at least one cooling channel, the at least one cooling channel is configured to receive a flow of fluid to be cooled. At least one first coolant flow duct that is configured to receive a flow of a first coolant, wherein the at least one cooling channel is disposed between a first inlet and a first outlet. The heat exchanger assembly further include at least one second coolant flow duct that is configured to receive a flow of a second coolant, wherein the at least one cooling channel is disposed between a second inlet and a second outlet.

Abstract: An engine component assembly includes a first engine component having a hot surface in thermal communication with a hot combustion gas flow and a cooling surface with at least one cavity. A second engine component is spaced from the cooling surface, and includes at least one cooling aperture. The cooling aperture is arranged such that cooling fluid impinges on the cooling surface at an angle.