Abstract: This invention relates to methods and reagents for detecting the presence of symmetrically and asymmetrically methylated arginine residues, either along or in polypeptides, using mass spectrometry. It also provides methods of determining structures of methylated arginine residues, methods of identifying substrates of PMRTs, and methods of conducting proteomic business using any of the suitable methods recited above.

Abstract: Methods and reagents for analyzing differential expression and/or abundance of distinct membrane-associated polypeptide samples, particularly integral membrane polypeptide samples are provided. Also provided are methods for screening pharmaceutical components that can affect expression or abundance of certain membrane-associated polypeptides; methods for identification of drug targets; and methods for diagnosis of certain disease states. Business methods for conducting a pharmaceutical business based on the result of using the above methods are also provided.

Abstract: This invention relates to methods and reagents for detecting the presence of symmetrically and asymmetrically methylated arginine residues, either along or in polypeptides, using mass spectrometry. It also provides methods of determining structures of methylated arginine residues, methods of identifying substrates of PMRTs, and methods of conducting proteomic business using any of the suitable methods recited above.