Abstract: An apparatus (e.g., for cell culture for in vitro growth of tissues and/or organs) includes a base, a plate on the base, and a removable barrier component on the plate. The removable barrier component is configured to at least partially define a first region. The removable barrier component includes a bottom surface and a top surface. The bottom surface configured to be in contact with the plate. The top surface is opposite the bottom surface. The press is configured to engage the top surface of the removable barrier component to apply a predetermined pressure to the removable barrier component. The removable barrier component is between the press and the plate. A joint between the plate and the removable barrier component may be substantially impermeable to live cells. The apparatus may further include a removable cover that engages the base to enclose the plate, the removable barrier component, and the press.

Abstract: In accordance with some preferred embodiments, without limitation, the present invention comprises compositions, methods and systems for preparation of stem-cell enriched cell populations from sources of biological materials by sorting cell types in relation to size.

Abstract: The present invention discloses a composite made of a layer of epithelial cells cultured together with a dermal matrix in a culture medium, which is free of BPE. The dermal matrix is a substantially non-immunogenic human dermis. Also disclosed is an enhanced composite where a biological agent has been introduced into the epithelial cells. The invention also comprises methods of making and implanting both the composite and the enhanced composite in an animal. A method of treating disease or injury through the use of the disclosed composites is also part of the present invention.