Abstract: A membrane capsule for biological and chemical separations comprising a cassette comprising an upper surface and a lower surface adjoined by a cassette sidewall, an inlet and an outlet located on the upper and lower surfaces of the cassette, tubes fluidly connected to the inlet and the outlet, holes or slots in the tubes to facilitate separation, and a membrane wrapped, pleated, and/or spiral wound around each of the tubes. Methods of separation comprising flowing fluid flow through the inlet of the membrane capsule, allowing the fluid to permeate through the holes or slots of the tubes, separating biological and/or non-biological substances, collecting the fluid within a reservoir, and draining fluid from the reservoir.