Abstract: The present invention features a method of producing an immunogenic composition against a pathogen, wherein the pathogen is a virus or bacterium. Also provided is an immunogenic composition produced thereof, as well as methods of use comprising said immunogenic composition.

Abstract: Gene therapy vectors can include a cytomegalovirus vector encoding one or more therapeutic donor genes. These vectors can be used in exemplary gene therapy methods for maintaining or improving one or more aspects of a recipient's physiological wellness and/or longevity. The recombinant viral vector can be administered or received intranasally or as an injectable therapeutic (singly or as a serial set of administrations) to beneficially cause one or more of the following salubrious effects in the patient: increased longevity, inhibited muscle degeneration, increases mitochondrial health, prevention of age-related hair loss, and/or increased blood glucose tolerance.

Abstract: The present invention is directed to a novel B. microti-based promoter and expression system. The present invention is also directed to a novel method of transfecting B. microti by electroporation, the resulting transfected B. microti parasite cell lines, and optionally, B. microti transfected with a particular novel promoter. In some embodiments, transgenic B. microti parasites that express reporter genes and their uses are provided. In certain embodiments, a multi-functional promoter controls multiple genes, e.g. a bifunctional promoter may control a gene of interest and a reporter or selection gene. The promoter may be particularly useful to control expression of apicomplexan genes and form apicomplexan proteins, e.g., without limitation, those of Babesia parasites. In embodiments, expression systems comprising the promoter are provided. In certain embodiments, B. microti-based systems that express reporter genes are provided.