Abstract: A method for promoting enzymolysis of lignocellulose by using a pH-responsive lignin amphoteric surfactant and recovery of a cellulase. The method includes: adding lignocellulose to a buffer solution, and then adding a pH-responsive lignin amphoteric surfactant and a cellulase; controlling the pH of the mixed solution to be 4.0-6.2, heating the solution to a temperature of 40° C. to 60° C. and reacting at said temperature for 24-96 h to obtain a saccharified hydrolyzate of lignocellulose; and obtaining an enzymolysed liquid by solid-liquid separation, and then adjusting the pH of the enzymolysed liquid to precipitate the pH-responsive lignin amphoteric surfactant and the cellulase for recycling. The method can effectively improve the enzymolysis efficiency of lignocellulose, recover a certain amount of cellulase, and expand the applications of industrial lignin. The method does not require additional equipment, and is simple to operate and environmentally friendly.

Abstract: A method for promoting enzymolysis of lignocellulose by using a pH-responsive lignin amphoteric surfactant and recovery of a cellulase. The method includes: adding lignocellulose to a buffer solution, and then adding a pH-responsive lignin amphoteric surfactant and a cellulase; controlling the pH of the mixed solution to be 4.0-6.2, heating the solution to a temperature of 40° C. to 60° C. and reacting at said temperature for 24-96 h to obtain a saccharified hydrolyzate of lignocellulose; and obtaining an enzymolysed liquid by solid-liquid separation, and then adjusting the pH of the enzymolysed liquid to precipitate the pH-responsive lignin amphoteric surfactant and the cellulase for recycling. The method can effectively improve the enzymolysis efficiency of lignocellulose, recover a certain amount of cellulase, and expand the applications of industrial lignin. The method does not require additional equipment, and is simple to operate and environmentally friendly.