Abstract: Disclosed herein is a skin condition-improving composition for topical application to the skin, comprising human growth hormone as an active ingredient, and a method for improving skin conditions using the same. The disclosed composition exhibits various skin conditioning effects, such as acne treatment, wrinkle improvement, dark spot removal, skin elasticity improvement, hair growth stimulation, skin aging prevention, skin moisturization and the proliferation of skin epidermal stem cells.

Abstract: Preparing a nanoliposome for encapsulating a protein includes preparing a dispersion by dispersing a phospholipid in an aqueous solution containing the protein, applying a shearing force to the dispersion; adding an additional amount of the phospholipid to the result of the step of applying the shearing force to the dispersion and then applying a shearing force higher than that of the step of applying the shearing force to the dispersion, and repeating the step of adding the additional amount of the phospholipid and applying the higher shearing force with an additional amount of the phospholipid and a shearing force higher than the prior step to obtain a nanoliposome having a desired diameter and encapsulation efficiency.

Abstract: An apparatus and a method for the submicronization of proteins using supercritical fluids is provided for feeding the supercritical fluid and feeding a protein solution using a precipitation vessel with a taper shape at its lower part, in which the precipitation vessel accommodates the supercritical fluid and the protein solution to generate submicroparticles of the protein, and a spray nozzle with coaxial arrangement having an outer nozzle for spraying the supercritical fluid and an inner nozzle for spraying the protein solution.