Abstract: Provided is a technique for preventing the adhesion of marine organisms to marine vessels, undersea structures, fishing gears, etc. over a long period of time, in particular, an environmentally-safe antifouling coating which makes it possible to prevent the adhesion of large-sized marine organisms. An antifouling coating including an antimicrobial composition each containing a nitrile-based antimicrobial agent, a pyridine-based antimicrobial agent, a haloalkylthio-based antimicrobial agent, an organic iodo-based antimicrobial agent, a thiazole-based antimicrobial agent, and a benzimidazole-based antimicrobial agent; an inorganic copper compound; a silicone-acrylic resin in which a content of silicone is 3 to 15 mass %; and a silicone oil is applied to marine vessels, undersea structures, fishing gears, etc.