Abstract: Even for the case where cells such as human epidermal keratinocytes form a dense colony, or the case where cell contours are indefinite, each of the cells is automatically tracked with high precision, and behavior of each cell is analyzed with good precision. There is provided a method for analyzing behavior of a cell, which comprises a detection step of detecting positions of a plurality of cells for every frame of time-lapse images, while determining whether a candidate region extracted from the frame is a cell region by using a dictionary containing image data of cell nuclei; and a tracking step of tracking each cell by using a state space model using position of a most adjacent cell within a predetermined distance from a predicted position as observation data. When any cell is not found within a certain distance from the predicted position, data are considered missing.

Abstract: Even for the case where cells such as human epidermal keratinocytes form a dense colony, or the case where cell contours are indefinite, each of the cells is automatically tracked with high precision, and behavior of each cell is analyzed with good precision. There is provided a method for analyzing behavior of a cell, which comprises a detection step of detecting positions of a plurality of cells for every frame of time-lapse images, while determining whether a candidate region extracted from the frame is a cell region by using a dictionary containing image data of cell nuclei; and a tracking step of tracking each cell by using a state space model using position of a most adjacent cell within a predetermined distance from a predicted position as observation data. When any cell is not found within a certain distance from the predicted position, data are considered missing.

Abstract: The present invention provides a method for screening a cytostatic agent characterized by determining an effect of inhibiting intracellular signaling mediated by a remaining fragment of a cell membrane-anchored growth factor, and the cytostatic agent. In growth factors of the EGF family, the physiological significance of the remaining fragment is clarified, and a method for controlling its action is searched, and thus a novel pharmaceutical can be provided.

Abstract: The present invention provides a method for screening a cytostatic agent characterized by determining an effect of inhibiting intracellular signaling mediated by a remaining fragment of a cell membrane-anchored growth factor, and the cytostatic agent. In growth factors of the EGF family, the physiological significance of the remaining fragment is clarified, and a method for controlling its action is searched, and thus a novel pharmaceutical can be provided.