Patents by Inventor Daisuke Yokouchi

Daisuke Yokouchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Botulinum toxin producing method
    Patent number: 11926853
    Abstract: Provided is a botulinum toxin producing method which is simple achieves a high toxin yield, and obtains a toxin having high specific activity. This botulinum toxin producing method includes: (A) a step in which a botulinum toxin is produced from botulinum toxin-producing bacteria in a medium, and a mixture a is obtained which contains a botulinum toxin, a bacterial component, and a nucleic acid component derived from the botulinum toxin; (B) a step in which the mixture a is subjected to the removal of the bacterial component, and a mixture b is obtained which contains a nucleic acid component and a botulinum toxin; (C) a step in which an endonuclease is added to the mixture b and a mixture c is obtained which contains a nucleic acid degradation product and a botulinum toxin; and (D) a step in which the mixture c is subjected to removal of the nucleic acid degradation product, and an isolated botulinum toxin liquid d is obtained.
    Type: Grant
    Filed: December 17, 2019
    Date of Patent: March 12, 2024
    Assignee: The Research Foundation For Microbial Diseases of Osaka University
    Inventors: Daisuke Yokouchi, Akiko Miura, Ryu Okada, Yuzo Yamashita, Yuya Sasaki, Shogo Nishihata
  • BOTULINUM TOXIN PRODUCING METHOD
    Publication number: 20220081682
    Abstract: Provided is a botulinum toxin producing method which is simple achieves a high toxin yield, and obtains a toxin having high specific activity. This botulinum toxin producing method includes: (A) a step in which a botulinum toxin is produced from botulinum toxin-producing bacteria in a medium, and a mixture a is obtained which contains a botulinum toxin, a bacterial component, and a nucleic acid component derived from the botulinum toxin; (B) a step in which the mixture a is subjected to the removal of the bacterial component, and a mixture b is obtained which contains a nucleic acid component and a botulinum toxin; (C) a step in which an endonuclease is added to the mixture b and a mixture c is obtained which contains a nucleic acid degradation product and a botulinum toxin; and (D) a step in which the mixture c is subjected to removal of the nucleic acid degradation product, and an isolated botulinum toxin liquid d is obtained.
    Type: Application
    Filed: December 17, 2019
    Publication date: March 17, 2022
    Inventors: Daisuke Yokouchi, Akiko Miura, Ryu Okada, Yuzo Yamashita, Yuya Sasaki, Shogo Nishihata