Abstract: A display substrate and a display device are provided. The display substrate includes a base substrate, sub-pixels and an isolation structure, each sub-pixel includes a light-emitting functional layer including film layers. The isolation structure is between adjacent sub-pixels, the isolation structure includes a first sub-isolation structure and a second sub-isolation structure, the first sub-isolation structure is between the second sub-isolation structure and the base substrate, and the size of the first sub-isolation structure is smaller than that of the second sub-isolation structure, the second sub-isolation structure includes a protruding portion; or, a slope angle of a side surface of the first sub-isolation structure is greater than 60 degrees and less than 120 degrees, and/or, a slope angle of a side surface of the second sub-isolation structure is greater than 60 degrees and less than 120 degrees; and at least one film layer is disconnected at the isolation structure.

Abstract: The disclosure provides a display substrate motherboard, a manufacturing method thereof, a display panel motherboard and a manufacturing method of a display substrate. The display substrate motherboard includes a base substrate including multiple substrate areas, each substrate area including a display region and a pad region; a display structure layer located on the base substrate and including a pixel defining layer and multiple film layers located between the pixel defining layer and the base substrate, a blocking region is arranged between any two adjacent substrate areas; a portion of the display structure layer in the blocking region has a first groove therein, a portion of the display structure layer between the first groove and the pad region adjacent thereto constitutes a first spacer region; a thickness of the portion of the display structure layer in the first spacer region is less than that in the display region.

Abstract: The disclosure provides a display substrate motherboard, a manufacturing method thereof, a display panel motherboard and a manufacturing method of a display substrate. The display substrate motherboard includes a base substrate including multiple substrate areas, each substrate area including a display region and a pad region; a display structure layer located on the base substrate and including a pixel defining layer and multiple film layers located between the pixel defining layer and the base substrate, a blocking region is arranged between any two adjacent substrate areas; a portion of the display structure layer in the blocking region has a first groove therein, a portion of the display structure layer between the first groove and the pad region adjacent thereto constitutes a first spacer region; a thickness of the portion of the display structure layer in the first spacer region is less than that in the display region.