Abstract: An improved dental floss holder having a comparatively inflexible elongate member supporting two flexible spaced arms at the opposing ends, and supporting an inflexible spaced arm between. A single length of commercially available dental floss is fastened near the terminal end of either flexible arm, then wrapped around the inflexible spaced arm, then fastened at the flexible spaced arm at the opposite end; two taut and usable lengths of dental floss are thus defined, either of which may be used for flossing while the other end of the dental floss holder is used as a handle. The dental floss fastening systems of the invention include buttons/protrusions in combination with frictional securing slots.