Abstract: Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with sample fluidic lines and a filter packaged in a disposable tip which concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. The concentrate is then dispensed from the disposable tip in a set volume of elution fluid. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect and identify. A single-use pipette tip includes fluid ports for aspirating the sample and connecting to a concentrating unit.

Abstract: The present disclosure provides for devices, systems and methods for fractionation and concentration of particles from a fluid sample. This includes a cartridge containing staged filters having porous surface in series of decreasing pore size for capture of particles from a fluid sample; and a permeate pressure source in fluid communication with the cartridge; wherein the particles are eluted from the porous surfaces and dispensed in a reduced fluid volume.

Abstract: The present disclosure provides for devices, systems and methods for fractionation and concentration of particles from a fluid sample. This includes a cartridge containing staged filters having porous surface in series of decreasing pore size for capture of particles from a fluid sample; and a permeate pressure source in fluid communication with the cartridge; wherein the particles are eluted from the porous surfaces and dispensed in a reduced fluid volume.

Abstract: Biological compositions containing animal fats and plant oils desirably are free of contaminants prior to processing into a biofuel. Disclosed herein is a method of removing such contaminants from these compositions to make that processing more efficient. The method employs a unique arrangement of mixers and centrifuges along with acidic solutions and recycle streams to remove these contaminants from the compositions.

Abstract: Biological compositions containing animal fats and plant oils desirably are free of contaminants prior to processing into a biofuel. Disclosed herein is a method of removing such contaminants from these compositions to make that processing more efficient. The method employs a unique arrangement of mixers and centrifuges along with acidic solutions and recycle streams to remove these contaminants from the compositions.

Abstract: Biological compositions containing animal fats and plant oils desirably are free of contaminants prior to processing into a biofuel. Disclosed herein is a method of removing such contaminants from these compositions to make that processing more efficient. The method employs a unique arrangement of mixers and centrifuges along with acidic solutions and recycle streams to re-move these contaminants from the compositions.

Abstract: Biological compositions containing animal fats and plant oils desirably are free of contaminants prior to processing into a biofuel. Disclosed herein is a method of removing such contaminants from these compositions to make that processing more efficient. The method employs a unique arrangement of mixers and centrifuges along with acidic solutions and recycle streams to re move these contaminants from the compositions.

Abstract: Systems and methods for managing certificates are described. A system comprises modules to receive requests related to a digital certificate, receive responses related to authorization of the digital certificate related to the requests, and store the requests and responses. A system comprises modules to communicate audit requests, receive responses related to the audit requests, and store the responses. A system comprises modules configured to communicate audit requests related to different certificate authorization entities, receive responses related to the audit requests, and store the responses. Other embodiments are described.

Abstract: The present disclosure provides for devices, systems and methods for fractionation and concentration of particles from a fluid sample. This includes a cartridge containing staged filters having porous surface in series of decreasing pore size for capture of particles from a fluid sample; and a permeate pressure source in fluid communication with the cartridge; wherein the particles are eluted from the porous surfaces and dispensed in a reduced fluid volume.

Abstract: Systems and methods for managing certificates are described. A system comprises modules to receive requests related to a digital certificate, receive responses related to authorization of the digital certificate related to the requests, and store the requests and responses. A system comprises modules to communicate audit requests, receive responses related to the audit requests, and store the responses. A system comprises modules configured to communicate audit requests related to different certificate authorization entities, receive responses related to the audit requests, and store the responses. Other embodiments are described.

Abstract: This invention relates to a method of sealing a cylinder head shaped to fit against an end of a cylinder liner, to the cylinder liner by creating an annular ring on the cylinder side of the cylinder head, creating a matching a annular rebate in the cylinder head end of the cylinder liner, screw threading the ring and rebate and screwing the cylinder head and cylinder liner together. An interference fit on a separate portion of the ring and rebate seals the cylinder head and cylinder liner as they are screwed together.