Patents by Inventor Dalip Sethi

Dalip Sethi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250090979
    Abstract: A filter includes a base material made of a porous polyurethane having a plurality of micropores and an active group fixed to a surface of the base material. The active group is capable of specifically binding to a separation target.
    Type: Application
    Filed: December 3, 2024
    Publication date: March 20, 2025
    Inventors: Masatsugu IGARASHI, Norihiko Takeda, Dalip Sethi
  • Publication number: 20250092362
    Abstract: A method for preparing chimeric antigen receptor cells using a cell expansion system includes introducing target cells to a bioreactor of the cell expansion system, where the introduction of the target cells includes isolating the target cells from a source. The isolation of the target cells from the source includes contacting the source to one or more identifying component and then causing the source to move through a separating column, where the separating column disposed in the cell expansion system in line with the bioreactor. The method further includes introducing viral vectors to the bioreactor, introducing a transduction reagent to the bioreactor, and removing non-cellular material from the bioreactor by causing material in the bioreactor to flow through a bypass loop, where the bypass loop includes one or more size exclusion filters and the non-cellular material includes unused viral vectors and unused identifying components.
    Type: Application
    Filed: September 3, 2024
    Publication date: March 20, 2025
    Inventors: Dalip Sethi, Mindy M. Miller, Dennis J. Hlavinka
  • Publication number: 20250091032
    Abstract: A separation device for separating a separation target from a liquid includes a pump, a first valve, a second valve, and a controller. The pump is configured to cause a first liquid containing an antibody or an aptamer capable of specifically binding to an antigen of the separation target and a second liquid containing the separation target to pass through a base material made of a porous polyester or a porous polyurethane capable of binding to the antibody or the aptamer. The first valve may be configured to open and close a first flow path through which the first liquid flows. The second valve may be configured to open and close a second flow path through which the second liquid flows. The controller is configured to control operation of the pump, opening and closing of the first valve, and opening and closing of the second valve.
    Type: Application
    Filed: December 3, 2024
    Publication date: March 20, 2025
    Inventors: Masatsugu IGARASHI, Norihiko TAKEDA, Dalip SETHI
  • Publication number: 20240376430
    Abstract: A method for producing T-cells using a cell expansion system includes expanding T-cells using a small bioreactor of the cell expansion system, the small bioreactor having a surface area of about 2,000 cm2 and an intracapillary volume of about 58 milliliters. The method further includes causing T-cells to flow into a small bioreactor of the cell expansion system. A rate at which the T-cells flow into the small bioreactor may be greater than or equal to about 0.007 ?L/min/fiber to less than or equal to about 0.0281 ?L/min/fiber. The cell expansion system may further include a tubing set that is in fluid communication with the small bioreactor and a volume ratio of the tubing set to the bioreactor may be about 2.96.
    Type: Application
    Filed: May 7, 2024
    Publication date: November 14, 2024
    Inventors: Dalip SETHI, Mindy M. MILLER, Nathan SCHWAB, Nathan D. FRANK, Mark E. JONES
  • Patent number: 12043823
    Abstract: Implementations are described that relate to methods and systems for growing cells in a hollow fiber bioreactor. In implementations, the cells may be exposed to a number of growth factors including a combination of recombinant growth factors. In other implementations, the cells may be grown in co-culture with other cells, e.g., hMSC's. In implementations, the cells may include CD34+ cells. A coated membrane includes a membrane having a first coating configured to promote cellular adhesion to the membrane and a second coating that includes a soluble protein moiety.
    Type: Grant
    Filed: March 23, 2022
    Date of Patent: July 23, 2024
    Assignee: Terumo BCT, Inc.
    Inventors: Mark E. Jones, Dalip Sethi, Dennis J. Hlavinka, Thomas J. Felt
  • Publication number: 20240076597
    Abstract: A method for functionalizing a hollow-fiber membrane for cell expansion of targeted cells (e.g., natural killer cells) includes contacting a biotinylating molecule to a surface of the hollow-fiber membrane including an extracellular matrix component, the biotinylating molecule binding to the extracellular matrix component and having an affinity for the targeted cells. The biotinylated molecule may be selected from the group consisting of: cytokine, epitope, ligand, monoclonal antibody, stains, aptamer, and combinations thereof. The extracellular matrix component may be selected from the group consisting of: fibronectin, vitronectin, fibrinogen, collagen, laminin, and combinations thereof.
    Type: Application
    Filed: August 16, 2023
    Publication date: March 7, 2024
    Applicant: Terumo BCT, Inc.
    Inventors: Mark E. JONES, Nathan D. FRANK, Mindy M. MILLER, Ann Marie W. CUNNINGHAM, Dalip SETHI
  • Publication number: 20240060030
    Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.
    Type: Application
    Filed: August 11, 2023
    Publication date: February 22, 2024
    Inventors: Dalip SETHI, James ARTHUR, David Alan HANNA, Daniel CLEMENT, Brian J. NANKERVIS, Jerome HEINTZ, Nathan D. FRANK, Brett BAEVERSTAD, Dennis J. HLAVINKA
  • Publication number: 20230213514
    Abstract: Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixtu
    Type: Application
    Filed: February 17, 2023
    Publication date: July 6, 2023
    Inventors: William Busa, Philip H. Coelho, Jonathan Ellis, Dalip Sethi
  • Patent number: 11604191
    Abstract: Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixtu
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: March 14, 2023
    Assignee: THERMOGENESIS CORPORATION
    Inventors: William Busa, Philip H. Coelho, Jonathan Ellis, Dalip Sethi
  • Publication number: 20220306978
    Abstract: Implementations are described that relate to methods and systems for growing cells in a hollow fiber bioreactor. In implementations, the cells may be exposed to a number of growth factors including a combination of recombinant growth factors. In other implementations, the cells may be grown in co-culture with other cells, e.g., hMSC's. In implementations, the cells may include CD34+ cells. A coated membrane includes a membrane having a first coating configured to promote cellular adhesion to the membrane and a second coating that includes a soluble protein moiety.
    Type: Application
    Filed: March 23, 2022
    Publication date: September 29, 2022
    Inventors: Mark E. Jones, Dalip Sethi, Dennis J. Hlavinka, Thomas J. Felt
  • Patent number: 11141435
    Abstract: Disclosed herein are methods for contacting in a closed container a host liquid including target cells, microbubble reagents comprising gas-core lipid-shelled microbubbles, and one or more antibodies or other ligands that bind to cell surface molecules on the target cells, wherein the one or more antibodies or other ligands are bound to the target cells or the microbubbles, wherein the contacting under conditions to produce target cells linked to microbubbles via the one or more antibodies or other ligands and activating the target cells to generate activated target cells.
    Type: Grant
    Filed: March 14, 2019
    Date of Patent: October 12, 2021
    Assignee: ThermoGenesis Corporation
    Inventors: Philip H. Coelho, William Busa, Jonathan Ellis, Dalip Sethi
  • Publication number: 20200072834
    Abstract: Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixtu
    Type: Application
    Filed: August 30, 2019
    Publication date: March 5, 2020
    Inventors: William Busa, Philip H. Coelho, Jonathan Ellis, Dalip Sethi
  • Publication number: 20190345430
    Abstract: The present disclosure relates to completely closed systems suitable for bio-processing of cellular samples, for example peripheral blood samples used for immunotherapy applications, and related methods of use. The systems are not open to the air, thus allowing for sterile sample processing and transfer of the sample throughout the entirety of bio-processing. Each component of the disclosed systems contains a unique identifier, allowing for traceability of the sample as it proceeds through the various steps involved in bio-processing. The identifier ultimately traces the sample back to the patient from which the sample was derived. Certain embodiments provide a unique freezing bag for long-term storage of cellular samples. The freezing bag has a unique identifier that allows for easy traceability and retrieval of a bio-archived sample and at least two ports, one for sample testing, another for sterile docking to a device that allows for delivery of its contents to a patient.
    Type: Application
    Filed: January 8, 2018
    Publication date: November 14, 2019
    Inventors: Dalip SETHI, Nicolas A. BRUQUE, Xiaochun XU
  • Publication number: 20190282619
    Abstract: Disclosed herein are methods for contacting in a closed container a host liquid including target cells, microbubble reagents comprising gas-core lipid-shelled microbubbles, and one or more antibodies or other ligands that bind to cell surface molecules on the target cells, wherein the one or more antibodies or other ligands are bound to the target cells or the microbubbles, wherein the contacting under conditions to produce target cells linked to microbubbles via the one or more antibodies or other ligands and activating the target cells to generate activated target cells.
    Type: Application
    Filed: March 14, 2019
    Publication date: September 19, 2019
    Inventors: Philip H. Coelho, William Busa, Jonathan Ellis, Dalip Sethi
  • Publication number: 20180271908
    Abstract: Disclosed herein are methods of ameliorating or inhibiting critical limb ischemia or a condition associated with critical limb ischemia in a subject, whereby such methods comprise identifying a subject having a peripheral vascular disease, peripheral arterial disease, critical limb ischemia or a condition associated with critical limb ischemia and providing to said subject a composition comprising: a cell population, wherein the cell population comprises bone marrow total nucleated cells and red blood cells, an anticoagulant, and autologous plasma, wherein the composition has a viscosity of 1.5 to 5.0 centipoise (cP) measured at 37° C., wherein the composition comprises a viable cell dose and, wherein said composition is administered to said subject intramuscularly through a standard terminally-ported cannula needle, or a cannula side-ported needle or catheter comprising a plurality of ports and a closed end.
    Type: Application
    Filed: April 28, 2016
    Publication date: September 27, 2018
    Inventors: Dalip SETHI, Bruce BAKER, Venkatesh PONEMONE, Kenneth Lee HARRIS, Markud D. LACHER, James HEISERMAN
  • Patent number: 9439930
    Abstract: The present invention relates to a method and composition for the aspiration, processing, testing and infusion of bone marrow derived stem cells, as an adjuvant treatment in cardiovascular disorders. More specifically, the invention provides for the methods and compositions for the aspiration, analysis, processing, infusate preparation and infusion of bone-marrow derived stem cells, particularly in a rapid point-of-care environment, wherein a centrifugal fractionation and optically monitored separation of the bone marrow yield desired cellular product in the desired concentration and viscosity.
    Type: Grant
    Filed: June 4, 2014
    Date of Patent: September 13, 2016
    Assignee: CESCA THERAPEUTICS, INC.
    Inventors: Kenneth Harris, Dalip Sethi, Venkatesh Ponemone, Gary R. Cohan
  • Patent number: 9402867
    Abstract: The present invention relates to a method and composition for the aspiration, processing, testing and infusion of bone marrow derived stem cells, as an adjuvant treatment in cardiovascular disorders. More specifically, the invention provides for the methods and compositions for the aspiration, analysis, processing, infusate preparation and infusion of bone-marrow derived stem cells, particularly in a rapid point-of-care environment, wherein a centrifugal fractionation and optically monitored separation of the bone marrow yield desired cellular product in the desired concentration and viscosity.
    Type: Grant
    Filed: June 5, 2014
    Date of Patent: August 2, 2016
    Assignee: Cesca Therapeutics, Inc.
    Inventors: Kenneth Harris, Dalip Sethi, Venkatesh Ponemone, Gary R. Cohan
  • Patent number: 9393269
    Abstract: The present invention relates to a method and composition for the aspiration, processing, testing and infusion of bone marrow derived stem cells, as an adjuvant treatment in cardiovascular disorders. More specifically, the invention provides for the methods and compositions for the aspiration, analysis, processing, infusate preparation and infusion of bone-marrow derived stem cells, particularly in a rapid point-of-care environment, wherein a centrifugal fractionation and optically monitored separation of the bone marrow yield desired cellular product in the desired concentration and viscosity.
    Type: Grant
    Filed: June 5, 2014
    Date of Patent: July 19, 2016
    Assignee: Cesca Therapeutics, Inc.
    Inventors: Kenneth Harris, Dalip Sethi, Venkatesh Ponemone, Gary R. Cohan
  • Publication number: 20140341861
    Abstract: The present invention relates to a method and composition for the aspiration, processing, testing and infusion of bone marrow derived stem cells, as an adjuvant treatment in cardiovascular disorders. More specifically, the invention provides for the methods and compositions for the aspiration, analysis, processing, infusate preparation and infusion of bone-marrow derived stem cells, particularly in a rapid point-of-care environment, wherein a centrifugal fractionation and optically monitored separation of the bone marrow yield desired cellular product in the desired concentration and viscosity.
    Type: Application
    Filed: June 4, 2014
    Publication date: November 20, 2014
    Inventors: Kenneth Harris, Dalip Sethi, Venkatesh Ponemone, Gary R. Cohan
  • Publication number: 20140286870
    Abstract: The present invention relates to a method and composition for the aspiration, processing, testing and infusion of bone marrow derived stem cells, as an adjuvant treatment in cardiovascular disorders. More specifically, the invention provides for the methods and compositions for the aspiration, analysis, processing, infusate preparation and infusion of bone-marrow derived stem cells, particularly in a rapid point-of-care environment, wherein a centrifugal fractionation and optically monitored separation of the bone marrow yield desired cellular product in the desired concentration and viscosity.
    Type: Application
    Filed: June 5, 2014
    Publication date: September 25, 2014
    Inventors: Kenneth Harris, Dalip Sethi, Venkatesh Ponemone, Gary R. Cohan