Abstract: An engineered DNA polymerase, with increased property for single molecule sequencing compared to a wild-type DNA polymerase, comprising a combination of mutation sites and functional domains, wherein the combination of mutation sites and functional domains includes thermostable mutation sites, low Kd mutation sites, exonuclease-deficient sites and DNA binding domains.

Abstract: An engineered DNA polymerase, with increased property for single molecule sequencing compared to a wild-type DNA polymerase, comprising a combination of mutation sites and functional domains, wherein the combination of mutation sites and functional domains includes thermostable mutation sites, low Kd mutation sites, exonuclease-deficient sites and DNA binding domains.

Abstract: A positive photoresist stripper composition includes a solvent system having solubility parameters which fall within a range from about 8.5 to about 15 in an amount which falls within a range from about 65% to about 98%. An amine is present in an amount which falls within a range from about 2% to about 25%. A fatty acid having 8 to 20 carbon atoms is present in an amount which falls within a range from about 0.1% to about 10% (all percents being by weight). The amount of the amine and of the fatty acid are selected to provide a pH which falls in a range from about 6 to about 9.5. Positive photoresist is stripped from a substrate by immersing the substrate in the aforementioned composition. Metal deposited on the substrate is not attached by the composition.