Abstract: An in vitro selection method is described which identifies oligonucleotide probes that discriminate amongst closely related nucleic acid sequences and which involves iterative hybridizations, including subtractive hybridization. Using the method, oligonucleotides are identified which can discriminate among human papilloma virus (HPV) subtypes. Corresponding methods and kits for the detection of nucleic acids are described, which methods and kits may be used in analytical, diagnostic, research and related applications.

Abstract: The present invention relates to a method of detecting genetic variation in individuals by PCR amplification of the locus of interest, transferring the resulting PCR products to a membrane filter, hybridizing with allele-specific-oligonucleotides (ASOs), and visualizing the results. Such a method is in the field of diagnostics, pharmacogenetics, cancer therapeutics, and drug metabolism. In particular, the present invention relates to the detection of genetic polymorphisms in gene encoding xenobiotics metabolizing enzymes CYP1A1, CYP2D6, CYP3A4, and NAT2.

Abstract: The present invention relates to a method of diagnosis or prognosis of tumor cells in a patient displaying a mutator phenotype in the tumor which comprises the steps of: a) obtaining a genomic DNA sample of the tumor; b) obtaining a genomic DNA sample of a tumor-free tissue of the patient; subjecting the DNA samples of steps a) and b) to amplification using primers which are flanking repeat pattern affected by a mutator phenotype or cold inter-Alu PCR followed by hybridization with a probe corresponding to an instability prone locus; d) subjecting the amplified fragments of step c) to electrophoretic fractionation on a polyacrylamide gel to determine the presence of a variation in band profile between tumor and tumor-free DNA indicating a genomic instability associated with a mutator phenotype; and e) employing means for comparing the allelic status of at least one instability prone locus in the DNA samples of step c).

Abstract: The present invention relates to a DNA amplification primer pair for the simultaneous amplification of multiple highly polymorphic genomic loci, which comprises R14B264/Q560mak (SEQ ID NO:1/SEQ ID NO:2). A method for the DNA fingerprinting identification of genetically related or unrelated individuals, which comprises the steps of: a) collecting genomic DNA sample of the individuals; b) performing DNA amplification of the DNA samples of step a) using the primer pair R14B264/Q560mak (SEQ ID NO:1/SEQ ID NO:2); and c) separating the amplified DNA segments of step b); whereby three markers of the genomic DNA of different size are amplified and serve as DNA fingerprinting of the individuals.