Patents by Inventor Dan Buzatu
Dan Buzatu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9194868Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: GrantFiled: August 17, 2009Date of Patent: November 24, 2015Assignees: The United States of America, Vivione Biosciences, LLCInventors: Dan A. Buzatu, Jon G. Wilkes, Ted A. Moskal, Bill Nevius, Jason T. Taylor, Randal K. Tucker, Melinda Miller, Shawn Ramsanoop
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Publication number: 20150276573Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: March 26, 2014Publication date: October 1, 2015Applicant: Vivione Biosciences, LLCInventors: Dan Buzatu, Jon Wilkes, Ted Moskal, Bill Nevius, Melinda Miller, Shawn Ramsaroop
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Publication number: 20150275264Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: March 26, 2014Publication date: October 1, 2015Applicant: Vivione Biosciences, LLCInventors: Dan Buzatu, Jon Wilkes, Ted Moskal, Bill Nevius, Melinda Miller, Shawn Ramsaroop
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Publication number: 20150275263Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: March 26, 2014Publication date: October 1, 2015Applicant: Vivione Biosciences, LLCInventors: Dan Buzatu, Jon Wilkes, Ted Moskal, Bill Nevius, Melinda Miller, Shawn Ramsaroop
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Patent number: 9103788Abstract: Methods for detecting one or more target bacteria in a test sample are provided. It is shown herein that photosensitizers combined with intense light exposure reduce fluorescing background due to non-bacterial particles. This permits detection of subsequently labeled target bacterial cells (e.g., using a fluorescently labeled antibody) against a largely black background. In particular examples, the methods include incubating the test sample in a growth medium that permits growth of bacteria present in the sample, contacting the sample with a photo-sensitizer; exposing the sample to light under conditions sufficient for the photo-sensitizer to photobleach contaminating non-bacterial particulates present in the sample. The bacteria can then be substantially separated from the sample, thereby generating an isolated bacterial sample. The method can also include contacting the isolated bacterial sample with a binding agent specific for the one or more target bacteria, and detecting the one or more target bacteria.Type: GrantFiled: November 30, 2012Date of Patent: August 11, 2015Assignees: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES, VIVIONE BIOSCIENCES, LLCInventors: Jon G. Wilkes, Dan Buzatu, Randal Tucker, Thaddeus John Moskal, Jr.
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Publication number: 20150065853Abstract: A system and method for preprocessing magnetic resonance spectroscopy (MRS) data of brain tissue for pattern-based diagnostics is disclosed. The MRS preprocessing system includes an MRS preprocessing module that executes an operation that normalizes MRS spectrum data, recalibrates and scales the normalized MRS spectrum data, and then renormalizes the scaled MRS spectrum data. The resulting preprocessed MRS data is used to assist in identifying abnormalities in tissues shown in MRS scans.Type: ApplicationFiled: September 5, 2014Publication date: March 5, 2015Applicant: The United States of America, as represented by the Secretary, Dept. of Health and Human ServicesInventors: Jon G. Wilkes, Dan A. Buzatu, Pierre Alusta, Bruce Pearce, Ryan M. Kretzer, Inessa IM, Richard D. Beger
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Patent number: 8880354Abstract: A system and method for preprocessing magnetic resonance spectroscopy (MRS) data of brain tissue for pattern-based diagnostics is disclosed. The MRS preprocessing system includes an MRS preprocessing module that executes an operation that normalizes MRS spectrum data, recalibrates and scales the normalized MRS spectrum data, and then renormalizes the scaled MRS spectrum data. The resulting preprocessed MRS data is used to assist in identifying abnormalities in tissues shown in MRS scans.Type: GrantFiled: November 12, 2010Date of Patent: November 4, 2014Assignee: The United States of America, as Represented by the Secretary, Department of Health and Human ServicesInventors: Jon G. Wilkes, Dan A. Buzatu, Pierre Alusta, Bruce Pearce, Ryan M. Kretzer, Inessa Im, Richard D. Beger
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Patent number: 8704169Abstract: Disclosed is a mass spectrometer for analyzing a sample that has or is suspected of having microorganisms. The disclosed mass spectrometer has been uniquely configured to include a sample platform which functions as a counter electrode or discharge electrode and a surface to provide the sample to be analyzed. The mass spectrometer also includes an ion source positioned adjacent to the sample platform for ionizing and volatizing molecules within the sample, wherein the sample platform and the ion source are positioned such that during operation of the mass spectrometer an electrical discharge takes place between the ion source and the sample platform. Also disclosed are methods for generating a mass spectrum profile/fingerprint of a sample. The methods include positioning a sample platform having a sample adjacent to an ion source.Type: GrantFiled: October 11, 2011Date of Patent: April 22, 2014Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Pierre Alusta, Cameron Dorey, William Ryan Parker, Jon G. Wilkes, Dan Buzatu
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Publication number: 20130131992Abstract: A system and method for preprocessing magnetic resonance spectroscopy (MRS) data of brain tissue for pattern-based diagnostics is disclosed. The MRS preprocessing system includes an MRS preprocessing module that executes an operation that normalizes MRS spectrum data, recalibrates and scales the normalized MRS spectrum data, and then renormalizes the scaled MRS spectrum data. The resulting preprocessed MRS data is used to assist in identifying abnormalities in tissues shown in MRS scans.Type: ApplicationFiled: November 12, 2010Publication date: May 23, 2013Applicant: The Government USA as Represented by the Secretary Dept. of Health and Human ServicesInventors: Jon G. Wilkes, Dan A. Buzatu, Pierre Alusta, Bruce Pearce, Ryan M. Kretzer, Inessa Im, Richard D. Beger
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Publication number: 20130087700Abstract: Disclosed is a mass spectrometer for analyzing a sample that has or is suspected of having microorganisms. The disclosed mass spectrometer has been uniquely configured to include a sample platform which functions as a counter electrode or discharge electrode and a surface to provide the sample to be analyzed. The mass spectrometer also includes an ion source positioned adjacent to the sample platform for ionizing and volatizing molecules within the sample, wherein the sample platform and the ion source are positioned such that during operation of the mass spectrometer an electrical discharge takes place between the ion source and the sample platform. Also disclosed are methods for generating a mass spectrum profile/fingerprint of a sample. The methods include positioning a sample platform having a sample adjacent to an ion source.Type: ApplicationFiled: October 11, 2011Publication date: April 11, 2013Inventors: Pierre Alusta, Cameron Dorey, William Ryan Parker, Jon G. Wilkes, Dan Buzatu
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Publication number: 20110217694Abstract: In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.Type: ApplicationFiled: August 17, 2009Publication date: September 8, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Ted A. Moskal, Bill Nevius, Jason T. Taylor, Randal K. Tucker, Melinda Miller, Shawn Ramsanoop
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Publication number: 20110085971Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: October 15, 2010Publication date: April 14, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Publication number: 20110085972Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: October 15, 2010Publication date: April 14, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birts, Richard Beger
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Publication number: 20110027174Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: October 15, 2010Publication date: February 3, 2011Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Patent number: 7824660Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: GrantFiled: April 30, 2008Date of Patent: November 2, 2010Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Patent number: 7684934Abstract: A method for reproducibly analyzing mass spectra from different sample sources is provided. The method deconvolutes the complex spectra by collapsing multiple peaks of different molecular mass that originate from the same molecular fragment into a single peak. The differences in molecular mass are apparent differences caused by different charge states of the fragment and/or different metal ion adducts and/or reactant products of one or more of the charge states. The deconvoluted spectrum is compared to a library of mass spectra acquired from samples of known identity to unambiguously determine the identity of one or more components of the sample undergoing analysis.Type: GrantFiled: June 7, 2004Date of Patent: March 23, 2010Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Alexandre Shvartsburg, Jon G. Wilkes, Paul Chiarelli, Ricky D. Holland, Dan A. Buzatu, Michael A. Beaudoin
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Patent number: 7608240Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: GrantFiled: December 6, 2004Date of Patent: October 27, 2009Assignee: Board of Trustees of the University of ArkansasInventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Biris, Richard Berger, Mark Diggs
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Publication number: 20090022655Abstract: The present invention provides a novel approach to cancer therapy and diagnostics that utilizes nanotubes and other similar nanostructures as both an indirect source of radiation therapy (BNCT), and as delivery vehicles for other types of radio- and chemo-therapeutic materials, as well as imaging agents for diagnostic purposes.Type: ApplicationFiled: April 30, 2008Publication date: January 22, 2009Inventors: Dan A. Buzatu, Jon G. Wilkes, Dwight Miller, Jerry A. Darsey, Tom Heinze, Alex Birls, Richard Beger
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Publication number: 20080017847Abstract: A molecular structure. In one embodiment, the molecular structure includes a nanotube formed with a plurality of carbon atoms having a first end, an opposite, second end, and a body portion defined therebetween, wherein the body portion has an interior surface defining a cavity, an opposite, exterior surface and a longitudinal axis therethrough the cavity, and a porphyrin molecule having a plurality of carbon atoms and a first plurality of hydrogen atoms, wherein at its original state the porphyrin molecule has a plurality of pyrrole units and each pyrrole unit is coupled to another pyrrole unit through a methine bridge so as to form a ring structure with a second plurality of hydrogen atoms positioned peripherally along the ring structure. The porphyrin molecule is chemically coupled to the interior surface of the nanotube such that at least one of the second plurality of hydrogen atoms positioned peripherally along the ring structure is replaced by a carbon atom of the nanotube.Type: ApplicationFiled: September 13, 2005Publication date: January 24, 2008Applicant: Board of Trustees of the University of ArkansasInventors: Jerry Darsey, Dan Buzatu, Freddy Nguyen
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Publication number: 20070269814Abstract: A method of determining the presence and level of microorganisms and/or chemicals in samples taken from generally any non-laboratory substance or environment. The method preferably comprises one or a combination of the steps of (a) prescreening for threshold levels of targeted microorganisms and/or (b) confirming the presence of targeted microorganisms or chemicals by mass spectrometry fingerprint analysis.Type: ApplicationFiled: November 10, 2006Publication date: November 22, 2007Applicant: LITMUS, L.L.C.Inventors: JON WILKES, DAN BUZATU, DWIGHT MILLER, DANIEL CURTIS, MARK DIGGS, RAJESH NAYAK, FATEMEH RAFII, JOHN SUTHERLAND, RANDAL TUCKER