Abstract: The invention relates to an arrangement for fluorescence amplification including a substrate, a fluorescence amplifier coating applied to the substrate, and a thin fluorescencable material which lies on the coating and emits light with the emission wavelength ?E when it is exposed to excitation light of an excitation wavelength ?A. The fluorescence amplifier coating includes an interference layer system of high-index and low-index dielectric layers, which reflects at least the excitation light. What is crucial for the design of the coating is that the fluorescencable material applied to the coating is arranged on the surface of the coating in the region of the maximum of the electric field amplitude of the standing wave with the excitation wavelength ?A, which is formed during exposure to the excitation light.

Abstract: A non-destructive method of determining the uniformity of a coating on a substrate comprises coating the substrate with a homogenous coating mixture comprising a labeled compound and a chemically functional compound to which other chemical moieties can be bound, and detecting the presence and locations of the labeled compound on the surface. The invention also relates to a surface for attachment of molecules comprising a substrate and a coating thereon of a homogeneous mixture of a labeled compound and a first chemically functional compound to which other chemical moieties can be bound.

Abstract: The present invention relates generally to an apparatus and kit in the microtiter plate format for improved multiplexed microarraying. The apparatus overcomes current limitations with conventional polymeric and glass bottom microtiter plates. The invention also relates to an apparatus for conducting multiple biomolecular assays comprising a low self-fluorescent coated substrate having an upper and lower surface. The substrate upper surface has a pattern comprising a plurality of wells. A flexible superstructure having a plurality of through holes (i.e. openings) in a pattern that is similar to and aligns with the wells on the substrate is removably adherable to the upper surface of the substrate. Preferably, the superstructure is adhesively attachable to the substrate. A tray supports the substrate and the superstructure and acts as an alignment jig.

Abstract: The invention relates to an arrangement for fluorescence amplification including a substrate, a fluorescence amplifier coating applied to the substrate, and a thin fluorescencable material which lies on the coating and emits light with the emission wavelength ?E when it is exposed to excitation light of an excitation wavelength ?A. The fluorescence amplifier coating includes an interference layer system of high-index and low-index dielectric layers, which reflects at least the excitation light. What is crucial for the design of the coating is that the fluorescencable material applied to the coating is arranged on the surface of the coating in the region of the maximum of the electric field amplitude of the standing wave with the excitation wavelength ?A, which is formed during exposure to the excitation light.

Abstract: The present invention relates to a substrate for attachment of biomolecules. The substrate is coated with a multiamino organosilane. If desired, the substrate can be further modified prior to coating with a multiamino organosilane. Optional surface modifications include coating the substrate with SiO2 or leaching with acid to form a SiO2 rich layer. DNA, nucleic acids, or any bimolecules can be attached to the coated substrates of the invention. Although a variety of substrates are contemplated, the preferred substrate is a low self-fluorescent glass.

Abstract: A non-destructive method of determining the uniformity of a coating on a substrate comprises coating the substrate with a homogenous coating mixture comprising a labeled compound and a chemically functional compound to which other chemical moieties can be bound, and detecting the presence and locations of the labeled compound on the surface. The invention also relates to a surface for attachment of molecules comprising a substrate and a coating thereon of a homogeneous mixture of a labeled compound and a first chemically functional compound to which other chemical moieties can be bound.

Abstract: The present invention relates to a substrate for attachment of biomolecules. The substrate is coated with a multiamino organosilane. If desired, the substrate can be further modified prior to coating with a multiamino organosilane. Optional surface modifications include coating the substrate with SiO2 or leaching with acid to form a SiO2 rich layer.