Abstract: The invention relates to cell biology and regenerative medicine, specifically to a culture method and application for porcine limbal epithelial stem cells. The method involves pretreatment of cells, coating of culture dishes, centrifugation, culturing, passaging, and identification by immunofluorescence staining. The cultured cells are applicable in repairing corneal diseases, including limbal stem cell deficiency. During repair, the cells can be combined with biological materials to form a stem cell engineering carrier for implantation at the injury site. The separation and culture method is simple, yields a large number of cells, and is suitable for use with porcine eyeballs. Limbal epithelial stem cells cultured in vitro form distinct cell clusters, referred to as stem cell islands. The obtained cells exhibit phenotypes including BMI1+, ?Np63?+, CK14+, and ABCG2+.

Abstract: The invention relates to bioinformatics technology and specifically to an analysis method of a spatial transcriptome-based 4D-printed stem cell scaffold for enhancing diabetic skin injury healing. Spatial transcriptome sequencing is performed on tissues from both the PBS group and the combined treatment group. Cell clustering and annotation identify main cell types, followed by visualization of their spatial distributions in both groups. The proportions of various cell types are statistically quantified and plotted. Cell subsets exhibiting significant differential gene expression are enriched and verified. Using a 4D-printed stem cell scaffold combined with a piRNA inhibitor and stem cell therapy, the method reveals spatial distribution and dynamic cellular changes during wound healing. It identifies precise cellular locations and gene expression patterns within tissues, providing critical insights into the biological processes by which biomaterials promote diabetic injury repair.

Abstract: The present invention relates to cell biology and regenerative medicine, specifically to a method for isolating and culturing porcine endometrial mesenchymal stem cells (PEMSCs) and their applications. PEMSCs are sourced from uterine tissue of pigs, providing a streamlined process yielding a substantial quantity of cells. Cultured PEMSCs exhibit CD34?, CD11b?, CD44+, and CD105+ phenotypic markers and can differentiate into osteocytes, adipocytes, and chondrocytes in vitro. The method employs type II collagenase to degrade extracellular matrix collagen and DNase I to break down DNA, reducing cell aggregation. This combination ensures efficient tissue digestion and high-quality single-cell suspensions. Applications include tissue repair for intrauterine adhesions, skin injuries, and vascular damage. The repair involves combining PEMSCs with biomaterials and implanting them into lesion sites.

Abstract: The present invention belongs to the field of biomedical technology, and specifically relates to a new application for piR-hsa-164586 and MYH9, based on the interaction between piR-hsa-164586 and MYH9: piR-hsa-164586 regulates the expression of MYH9 and promotes the metastasis of NSCLC, piR-hsa-164586 and MYH9 can be used as a therapeutic target for NSCLC, and have become a potential therapeutic drug, wherein the MYH9 is obtained by RNA pulldown assay and verified by protein mass spectrometry, molecular docking and RIP assays, the piR-hsa-164586 can positively regulate the expression of MYH9, and verified by performed qRT-PCR, western blotting and tissue immunofluorescence staining assays on the piR-hsa-164586 NC group, piR-hsa-164586 KD group, and piR-hsa-164586 OE group. The interaction between piR-hsa-164586 and MYH9 can up-regulate the expression of MYH9 in NSCLC cell lines, the verification experiment process was confirmed by Transwell experiment after knockdown of piR-hsa-164586 and MYH9 respectively.

Abstract: A method for preparing high-density magnesia-alumina spinel ceramic by low-temperature pressureless sintering, comprises: using MgAl2O4 powder as a raw material powder, adding calcium phosphate as a sintering aid and controlling the calcium element of the calcium phosphate to not exceed 500 ppm of the total mass of the raw material powder; and then performing pressureless sintering, thereby preparing a high-density magnesia-alumina spinel ceramic; the pressureless sintering includes normal pressure sintering or vacuum sintering.

Abstract: Provided is a vibration diaphragm, comprising a wireless transmission unit which is arranged on one side of the vibration diaphragm where a voice coil can be fixed, and is used for electrically connecting with the voice coil, wirelessly receiving an audio signal transmitted from an external terminal and processing the audio signal to obtain audio current, and inputting the audio current into the voice coil; and a wireless charging unit which is electrically connected with the wireless transmission unit, generating electric energy under an action of an external power supply module and inputting the electric energy into the wireless transmission unit. Also provided are a vibration assembly, a sound generator and a sound generator module. With integration of the receiving and processing of audio signal on the vibration diaphragm, long-distance routing of a voice coil lead of the voice coil is avoided, noise is reduced, and product quality is improved.

Abstract: An LED display screen controller (10, 31) and a display control system (30) are provided. The LED display screen controller (10, 31) includes: an embedded processor (11, 311); a first WiFi component (14, 313); a second WiFi component (15, 314); a wired network component (16, 315); a first Ethernet PHY chip (17, 316), connected between the wired network component (16, 315) and the embedded processor (11, 311); a programmable logic component (12, 312), connected to the embedded processor (11, 311); and an output port (18), connected to the programmable logic component (12, 312) and configured to connect an LED display screen (33). The embedded processor (11, 311) is configured to configure the first WiFi component (14, 313) to work in an AP mode, and the second WiFi component (15, 314) is adapted to work in a Station mode when the first WiFi component (14, 313) works in the AP mode.

Abstract: The invention relates to an anterior cervical plate, a lifter and an internal fixation system for use in ACAF. Fusion windows are formed at a head end and a tail end of the anterior cervical plate, wherein each of the fusion windows has a width of more than 8 mm, and a height of more than 10 mm. At least one elongated lifting groove is provided at a central longitudinal axis. It further comprises an auxiliary plate which covers a front side of the plate body when in use. The lifter comprises a lifting nail and a screwdriver. The lifting nail has a first travel control body and a second travel control body. The screwdriver is provided with a screwdriver sleeve head sleeved outside of the second travel control body and making the screwdriver to rotate with it. The internal fixation system comprises the anterior cervical plate and the lifter.

Abstract: The invention relates to an anterior cervical plate, a lifter and an internal fixation system for use in ACAF. Fusion windows are formed at a head end and a tail end of the anterior cervical plate, wherein each of the fusion windows has a width of more than 8 mm, and a height of more than 10 mm. At least one elongated lifting groove is provided at a central longitudinal axis. It further comprises an auxiliary plate which covers a front side of the plate body when in use. The lifter comprises a lifting nail and a screwdriver. The lifting nail has a first travel control body and a second travel control body. The screwdriver is provided with a screwdriver sleeve head sleeved outside of the second travel control body and making the screwdriver to rotate with it. The internal fixation system comprises the anterior cervical plate and the lifter.

Abstract: Disclosed is a method and device for processing Image, and an image transmitting system. The method includes that: data to be transmitted are processed according to a first resolution to obtain first image data, wherein the image resolution represented by each row of image data in the first image data is the first resolution, and the first resolution is the maximum resolution set by a system; and the first image data is folded to obtain second image data, wherein the number of rows of the second image data is greater than that of the first image data, the image resolution represented by each row of image data in the second image data is a second resolution, and the second resolution is less than the first resolution.

Abstract: The embodiments of the present disclosure disclose a media method, device and system for generating a media playback sequence. The method includes acquiring a plurality of media identifiers respectively corresponding to a plurality of media, and media playbacks times respectively corresponding to the plurality of media identifiers; comparing a maximum media playbacks time among the media playbacks times with a sum of remaining media playbacks times to obtain a determination result; and selecting, according to the determination result, one of a plurality of different generation methods to generate a media playback sequence for a media player to use to play the plurality of media back, wherein the media playback sequence includes the plurality of media identifiers, and the number of each of the media identifiers in the media playback sequence is equal to the media playbacks time corresponding to each of the media identifiers.

Abstract: At least some embodiments of the present disclosure disclose a program production method, a program production device, a program production system and a computer-readable medium. The program production method includes that: at least one medium is acquired; at least one playlist is generated according to the at least one medium, each of the at least one playlist being associated with the at least one medium; at least one program is generated according to the at least one playlist, each of the at least one program including a play region and the play region corresponding to the at least one playlist; and the play region is scheduled.

Abstract: Disclosed is a manufacturing method for a high silicon grain oriented electrical steel sheet, the silicon content of the high silicon grain oriented electrical steel is greater than 4 wt %, comprising the steps of: (1) performing decarburization annealing of a cold-rolled steel plate; (2) allowing high silicon alloy particles in a completely solid state to collide at a high speed with the surface of the decarburization annealed steel plate to be sprayed, thus forming a high silicon alloy coating on the surface of the steel plate to be sprayed; (3) coating a release agent and drying; and (4) annealing. The manufacturing method for the high silicon grain oriented electrical steel sheet of the present invention is inexpensive, and, the high silicon grain oriented electrical steel sheet produced is of stable quality and is provided with great magnetic performance.

Abstract: Disclosed is a method and device for processing Image, and an image transmitting system. The method includes that: data to be transmitted are processed according to a first resolution to obtain first image data, wherein the image resolution represented by each row of image data in the first image data is the first resolution, and the first resolution is the maximum resolution set by a system; and the first image data is folded to obtain second image data, wherein the number of rows of the second image data is greater than that of the first image data, the image resolution represented by each row of image data in the second image data is a second resolution, and the second resolution is less than the first resolution.

Abstract: The present disclosure discloses a method and device for controlling screen display. The method includes that: a rotation instruction is received; a system window of a screen is rotated according to the rotation instruction, wherein when the system window is rotated, the absolute position of a play window in the system window changes accordingly; the play window is translated to a preset position in the system window; and a display image in the translated play window is transmitted to the screen for display, wherein the screen displays the display image received by a receiving card.

Abstract: Provided are a method device (20, 70) and system (30, 40, 80) for configuring a display screen, for example, the method includes that a network connection request including initial display screen information is sent in a wireless manner after power-on self-test, a network connection is established with a main console (301, 801), the initial display screen information is acquired, the initial display screen information is displayed, the updating operation to position information in the initial display screen information is carried out by the main console (301, 801) to obtain target display screen information, and a coordinate assignment of the target display screen information is displayed to obtain display coordinate information (S61); the target display screen information and the display coordinate information are received, the position information is updated, and identification codes in the display coordinate information are associated with the initial display screen information, and is stored locally (S62)

Abstract: An LED display screen controller (10, 31) and a display control system (30) are provided. The LED display screen controller (10, 31) includes: an embedded processor (11, 311); a first WiFi component (14, 313); a second WiFi component (15, 314); a wired network component (16, 315); a first Ethernet PHY chip (17, 316), connected between the wired network component (16, 315) and the embedded processor (11, 311); a programmable logic component (12, 312), connected to the embedded processor (11, 311); and an output port (18), connected to the programmable logic component (12, 312) and configured to connect an LED display screen (33). The embedded processor (11, 311) is configured to configure the first WiFi component (14, 313) to work in an AP mode, and the second WiFi component (15, 314) is adapted to work in a Station mode when the first WiFi component (14, 313) works in the AP mode.

Abstract: An apparatus and a method for rapidly heating cold-rolled strip steel (10). The apparatus for rapidly heating cold-rolled strip steel (10) comprises a heating zone, a soaking zone, and a cooling zone, and the heating zone is sequentially divided into a first heating section (1), a second heating section (2), a third heating section (3), and a fourth heating section (4) along a moving direction of the strip steel (10) to be heated, the first heating section (1) and the fourth heating section (4) being radiant heating sections, and the second heating section (2) and the third heating section (3) being inductive heating sections. The method for rapidly heating cold-rolled strip steel (10) uses the apparatus for rapidly heating cold-rolled strip steel (10) to heat the strip steel (10).

Abstract: The present disclosure relates to a cervical vertebra anterior fixation and fusion system maintaining natural height and the fixing plate. The fixing plate is provided with a plate body, wherein the plate body is provided with windows, locking members and screw fixing holes to be matched with screws. A minimum distance between an upper side wall and a lower side wall of the window in a vertical axis direction is 3-8 mm; and a minimum distance between a left side wall and a right side wall of the window in a transverse axis direction is 12.1-18 mm. The present disclosure further relates to a method for performing anterior cervical intervertebral disc resection and fusion by applying the fixation and fusion system.