Abstract: Cellular samples such as cervical cells can be analyzed using a unique combination of markers that identify abnormal cells while subtracting out false positives that would otherwise be generated by, for example, normally proliferating cells and non-cellular debris. Dysplastic cervical cells can thus be identified quickly and accurately. An assay including analytical reagents and methods of their use can be employed in classifying cervical cytology specimens as being normal or abnormal. This assay is configured to be performed using any of several commonly available classes of laboratory instrumentation and upon the entire range of cervical cytology specimen preparations encountered in clinical practice. This assay is particularly intended for use in high volume screening environments, but also has both diagnostic and research applications.