Abstract: Bioreactors and methods for growing a chemoautotrophic culture of a microorganism, such as a hydrogen-oxidizing or carbon monoxide-oxidizing microorganism, are provided. The bioreactors and methods provide for enhanced growth and productivity of microorganisms that use gaseous sources of carbon and energy and provide an environment for carbon fixing to produce organic molecules of interest and/or biomass. The present bioreactors and methods achieve enhanced growth with respect to cell density, culture duration and/or growth rate, while maintaining safe operating conditions.

Abstract: A protein hydrolysate composition derived from a microorganism, such as a chemoautotrophic microorganism, and methods of preparing and using the same are provided. The protein hydrolysate composition may be produced sustainably through fixation of carbon dioxide from biogenic or atmospheric sources. The protein hydrolysate composition finds use in supplementing culture media for serum-free culturing of animal cells as well as for growing other types of cells such as probiotics and lactic acid bacteria. Thus, the present disclosure provides sustainable, humane processes for culturing cells for pharmaceutical and nutraceutical application as well as for human consumption as a food ingredient or product, including cultured meat.

Abstract: Structured food compositions, such as meat analogue compositions, which include protein products from microorganisms, such as microorganism-derived protein hydrolysates, are described. Methods of making such products are also described.

Abstract: Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.

Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.

Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.

Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.

Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.

Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.

Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.

Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

Abstract: An apparatus, system, and method are directed towards parsing and selectively encrypting different portions of data in real-time, decrypting the encrypted data in real-time, and passing the data to a media player on a client computer or other network capable device. Data in a network packet may be parsed into payload and non-payload portions. The payload portion of the packet data may then be examined to determine whether a predefined type of the data is recognized. For example, in one embodiment, the predefined data type may be media content. If the payload portion is recognized as a predefined data type, then it may be selectively encrypted. The selectively encrypted payload portion and non-payload portion of the packet may then be combined, such that the non-payload portion may be employed by firewalls, proxies, and/or NATs to route the packet towards the client computer or other network capable device.

Abstract: The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1-hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.

Abstract: An apparatus, system, and method are directed towards parsing and selectively encrypting different portions of data in real-time, decrypting the encrypted data in real-time, and passing the data to a media player on a client computer or other network capable device. Data in a network packet may be parsed into payload and non-payload portions. The payload portion of the packet data may then be examined to determine whether a predefined type of the data is recognized. For example, in one embodiment, the predefined data type may be media content. If the payload portion is recognized as a predefined data type, then it may be selectively encrypted. The selectively encrypted payload portion and non-payload portion of the packet may then be combined, such that the non-payload portion may be employed by firewalls, proxies, and/or NATs to route the packet towards the client computer or other network capable device.

Abstract: An apparatus and method for selectively encrypting portions of data sent over a network between a server and a client. The apparatus includes parsing means for separating a first portion of the data from a second portion of the data, encrypting means for encrypting only of the first portion of the data, and combining means for combining the encrypted first portion of the data with the second portion of the data, wherein the second portion of the data is not encrypted. The apparatus further includes decrypting means installed at the client for decrypting the encrypted portion of the data. The apparatus is platform independent in terms of media format and data protocol. The encryption unit encrypts data transparently to the client based on the media format. The apparatus of the invention is implemented as one of an application and a plug-in object.

Abstract: The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.

Abstract: The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and temperature.

Abstract: In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.