Patents by Inventor Dana M. Fowlkes
Dana M. Fowlkes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7611854Abstract: Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of ?-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.Type: GrantFiled: June 18, 2003Date of Patent: November 3, 2009Assignee: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, Jim Broach, John Manfredi, Christine Klein, Andrew J. Murphy, Jeremy Paul, Joshua Trueheart
-
Publication number: 20090264303Abstract: Novel polypeptides having functional domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.Type: ApplicationFiled: May 21, 2007Publication date: October 22, 2009Inventors: Andrew B. Sparks, Noah Hoffman, Brian K. Kay, Dana M. Fowlkes, Stephen J. McConnell
-
Publication number: 20080268475Abstract: The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and/or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast ? factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.Type: ApplicationFiled: April 21, 2008Publication date: October 30, 2008Applicant: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, James R. Broach, John P. Manfredi, Jeremy I. Paul, Joshua Trueheart, Christine A. Klein, Andrew J.M. Murphy
-
Patent number: 7416881Abstract: Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of ?-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.Type: GrantFiled: April 5, 1999Date of Patent: August 26, 2008Assignee: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, Jim Broach, John Manfredi, Christine Klein, Andrew J. Murphy, Jeremy Paul, Joshua Trueheart
-
Patent number: 7361498Abstract: The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and/or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast ? factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.Type: GrantFiled: October 15, 2004Date of Patent: April 22, 2008Assignee: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, James R. Broach, John P. Manfredi, Jeremy I. Paul, Joshua Trueheart, Christine A. Klein, Andrew J. M. Murphy
-
Patent number: 7279548Abstract: Novel polypeptides having WW domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.Type: GrantFiled: June 28, 2002Date of Patent: October 9, 2007Assignees: Cytogen Corporation, The University of North Carolina at Chapel HillInventors: Gregorio Pirozzi, Brian K. Kay, Dana M. Fowlkes
-
Patent number: 7250263Abstract: The present invention makes available a rapid, effective assay for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular receptor or ion channel. The subject assay enables rapid screening of large numbers of polypeptides in a library to identifying those polypeptides which induce or antagonize receptor bioactivity. The subject assay is particularly amenable for identifying surrogate ligands for orphan receptors.Type: GrantFiled: January 5, 2004Date of Patent: July 31, 2007Assignee: Cadus Technologies, Inc.Inventors: Christine A. Klein, Andrew J. M. Murphy, Dana M. Fowlkes, James Broach, John Manfredi, Jeremy Paul, Joshua Trueheart
-
Patent number: 7235648Abstract: Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of ?-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.Type: GrantFiled: February 26, 1999Date of Patent: June 26, 2007Assignee: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, Jim Broach, John Manfredi, Christine Klein, Andrew J. Murphy, Jeremy Paul, Joshua Trueheart
-
Patent number: 7223547Abstract: Novel polypeptides having functional domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.Type: GrantFiled: March 23, 2004Date of Patent: May 29, 2007Assignees: Cytogen Corporation, University of North Carolina at Chapel HillInventors: Andrew B. Sparks, Noah Hoffman, Brian K. Kay, Dana M. Fowlkes, Stephen J. McConnell
-
Patent number: 7122305Abstract: The present invention makes available a rapid, effective assay for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular receptor or ion channel. The subject assay enables rapid screening of large numbers of polypeptides in a library to identifying those polypeptides which induce or antagonize receptor bioactivity. The subject assay is particularly amenable for identifying surrogate ligands for orphan receptors.Type: GrantFiled: December 21, 2000Date of Patent: October 17, 2006Assignee: Cadus Technologies, Inc.Inventors: Christine A. Klein, Andrew J. Murphy, Dana M. Fowlkes, James Broach, John Manfredi, Jeremy Paul, Joshua Trueheart
-
Patent number: 7105309Abstract: Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of ?-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.Type: GrantFiled: October 1, 2002Date of Patent: September 12, 2006Assignee: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, James R. Broach, John P. Manfredi, Christine A. Klein, Andrew J. M. Murphy, Jeremy I. Paul, Joshua Trueheart
-
Patent number: 6864060Abstract: The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and/or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast ? factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.Type: GrantFiled: October 7, 1997Date of Patent: March 8, 2005Assignee: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, James R. Broach, John P. Manfredi, Jeremy I. Paul, Joshua Trueheart, Christine A. Klein, Andrew J. M. Murphy
-
Publication number: 20040197840Abstract: Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of &agr;-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.Type: ApplicationFiled: June 18, 2003Publication date: October 7, 2004Applicant: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, Jim Broach, John Manfredi, Christine Klein, Andrew J. Murphy, Jeremy Paul, Joshua Trueheart
-
Publication number: 20040157216Abstract: Novel polypeptides having functional domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.Type: ApplicationFiled: March 23, 2004Publication date: August 12, 2004Inventors: Andrew B. Sparks, Noah Hoffman, Brian K. Kay, Dana M. Fowlkes, Stephen J. McConnell
-
Patent number: 6709821Abstract: Novel polypeptides having functional domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.Type: GrantFiled: June 13, 2001Date of Patent: March 23, 2004Assignees: University of North Carolina at Chapel Hill, Cytogen Corp.Inventors: Andrew B. Sparks, Noah Hoffman, Brian K. Kay, Dana M. Fowlkes, Stephen J. McConnell
-
Publication number: 20030224390Abstract: Peptides which bind a cellular (surface or intracellular) receptor, such as a nuclear receptor, may be identified by screening a combinatorial peptide library presented in the form of cells each of which coexpress one member peptide and the receptor, together with a signal producing system for reporting binding. A “two-hybrid” assay is of particular interest. The screen may be carried out in the presence of a ligand, in particular, an exogenous ligand. If this screening is carried out for a plurality of different receptor conformations, then this library screening will also serve to identify conformation-specific peptides for the receptor, which may then be used in a panel for “fingerprinting” query compounds as to their ability to interact with the receptor in the presence of each of the panel peptides. These fingerprints may be compared to those of reference compounds with known biological activities mediated by that receptor.Type: ApplicationFiled: January 17, 2003Publication date: December 4, 2003Applicant: Karo Bio ABInventors: Dana M. Fowlkes, Thomas R. Barnett, Benjamin Buehrer
-
Publication number: 20030203417Abstract: Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of &agr;-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.Type: ApplicationFiled: October 1, 2002Publication date: October 30, 2003Inventors: Dana M. Fowlkes, James R. Broach, John P. Manfredi, Christine A. Klein, Andrew J.M. Murphy, Jeremy I. Paul, Joshua Trueheart
-
Publication number: 20030186863Abstract: Peptides having general and specific binding affinities for the Src homology region 3 (SH3) domains of proteins are disclosed in the present invention. In particular, SH3 binding peptides have been isolated from phage-displayed random peptide libraries which had been screened for isolates that bind to bacterial fusion proteins comprising SH3 and glutathione S-transferase (GST). Preferred peptides are disclosed which comprise a core 7-mer sequence (preferably, a consensus motif) and two or more, preferably at least six, additional amino acid residues flanking the core sequence, for a total length of 9, preferably at least 13, amino acid residues and no more than about 45 amino acid residues. Such peptides manifest preferential binding affinities for certain SH3 domains. The preferred peptides exhibit specific binding affinities for the Src-family of proteins. In vitro and in vivo results are presented which demonstrate the biochemical activity of such peptides.Type: ApplicationFiled: May 31, 2002Publication date: October 2, 2003Inventors: Andrew B. Sparks, Brian K. Kay, Judith M. Thorn, Lawrence A. Quilliam, Channing J. Der, Dana M. Fowlkes, James E. Rider
-
Patent number: 6617114Abstract: The present invention is directed to the identification of compounds in a compound library which can mediate the biological activity of a target receptor protein, even when the ligands which mediate that activity through binding to that receptor are not already known. The method of the invention includes the following three steps: (1) Screen a first combinatorial library for members binding to the target protein (TP) and hence capable of use as surrogates for the unknown ligand in steps (2) and (3). (2) Screen a second library, for compounds which inhibit the binding of one or more surrogates from step (1) to TP, and, optionally. (3) Determine whether the inhibitory compound mediates the biological activity of the said TP. The first library is composed of peptides, peptoids and/or nucleic acids, and the second is not.Type: GrantFiled: April 30, 1998Date of Patent: September 9, 2003Assignee: Karo Bio ABInventors: Dana M. Fowlkes, Brian K. Kay, Jeffrey A. Frelinger, Robin Parish Hyde-Deruyscher
-
Publication number: 20030077577Abstract: Novel polypeptides having WW domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.Type: ApplicationFiled: June 28, 2002Publication date: April 24, 2003Inventors: Gregorio Pirozzi, Brian K. Kay, Dana M. Fowlkes