Abstract: A device for dust removal through air curtain isolation and self-circulation purification, including a material input transferring cabin, a main isolation cabin, a material output transferring cabin and a control cabinet. By employing three independent interlayer isolation cabins, a self-circulation purification device formed of self-circulation air pipes is disposed in the main isolation cabin, and an airflow cleaning air curtain is respectively disposed between the main isolation cabin and the material input transferring cabin and also between the main isolation cabin and the material output transferring cabin. By means of air curtain isolation and self-circulation purification, the spreading of dust resulting in pollution and interference to the environment of both the processing area and the whole clean area can be avoided, which facilitates the cleanness of the pharmaceutical processing environment to meet the standard requirements in the Chinese GMP Version 2010.