Patents by Inventor Daniel A. Lorenz

Daniel A. Lorenz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods and use of chimeric proteins
    Patent number: 12584125
    Abstract: Provided are methods for using chimeric proteins to produce RNA modifications that can be detected by sequencing methods, including methods detecting relative translation rates of various mRNAs. Also provided herein are compositions comprising chimeric proteins, wherein the chimeric proteins comprise a RNA editing protein and a ribosomal protein.
    Type: Grant
    Filed: March 12, 2021
    Date of Patent: March 24, 2026
    Assignee: The Regents of the University of California
    Inventors: Eugene Yeo, Kristopher W. Brannan, Ryan Marina, Isaac A. Chaim, Daniel A. Lorenz
  • Methods for detecting RNA binding protein complexes
    Patent number: 12460257
    Abstract: The present disclosure relates to methods of identifying RNA targets of RNA binding proteins. In aspects, the disclosure relates to a method of identifying RNA molecules bound by RNA binding proteins. Some embodiments of the present disclosure relate to a method that can definitively identify direct RNA-target interactions with targeted proteins without the requirement for immunoprecipitation or gel extraction. In some embodiments, the method may include combining multiple antibodies in the same sample.
    Type: Grant
    Filed: July 25, 2023
    Date of Patent: November 4, 2025
    Assignee: Eclipse Bioinnovations, Inc.
    Inventors: Daniel A. Lorenz, Karen B. Chapman
  • METHODS FOR DETECTING RNA BINDING PROTEIN COMPLEXES
    Publication number: 20230357829
    Abstract: The present disclosure relates to methods of identifying RNA targets of RNA binding proteins. In aspects, the disclosure relates to a method of identifying RNA molecules bound by RNA binding proteins. Some embodiments of the present disclosure relate to a method that can definitively identify direct RNA-target interactions with targeted proteins without the requirement for immunoprecipitation or gel extraction. In some embodiments, the method may include combining multiple antibodies in the same sample.
    Type: Application
    Filed: July 25, 2023
    Publication date: November 9, 2023
    Inventors: Daniel A. Lorenz, Karen B. Chapman
  • Methods for detecting RNA binding protein complexes
    Patent number: 11795500
    Abstract: The present disclosure relates to methods of identifying RNA targets of RNA binding proteins. In aspects, the disclosure relates to a method of identifying RNA molecules bound by RNA binding proteins. Some embodiments of the present disclosure relate to a method that can definitively identify direct RNA-target interactions with targeted proteins without the requirement for immunoprecipitation or gel extraction. In some embodiments, the method may include combining multiple antibodies in the same sample.
    Type: Grant
    Filed: August 18, 2022
    Date of Patent: October 24, 2023
    Assignee: Eclipse Bioinnovations, Inc.
    Inventors: Daniel A. Lorenz, Karen B. Chapman
  • METHODS FOR OLIGO TARGETED PROXIMITY LIGATION
    Publication number: 20230313177
    Abstract: The present disclosure relates to methods of enriching for nucleic acid sequence using a targeting proximity-base ligation. In some embodiments, the method comprises preparing nucleic acid from a sample, complexing the nucleic acid from a sample with the one or more targeting oligonucleotides comprising a targeting region linked to a reverse transcription region, and preparing a library of nucleic acids amplified from the oligonucleotides. Further, kits are disclosed for preparing and producing the methods described herein.
    Type: Application
    Filed: February 23, 2023
    Publication date: October 5, 2023
    Inventors: Daniel A. Lorenz, Karen B. Chapman
  • METHODS FOR DETECTING RNA MODIFICATION TARGETS ON A GENE
    Publication number: 20230304076
    Abstract: The present disclosure relates to methods of identifying an RNA modification targets from a gene. In some embodiments, the method comprises preparing RNA from a sample, preparing one or more antibody conjugates comprising an antibody linked to an oligonucleotide, complexing the RNA from a sample with the one or more antibody conjugates, and preparing a library of nucleic acids amplified from the oligonucleotides. Further, kits are disclosed for preparing and producing the methods described herein.
    Type: Application
    Filed: December 29, 2022
    Publication date: September 28, 2023
    Inventors: Daniel A. Lorenz, Karen B. Chapman
  • METHODS FOR DETECTING RNA TRANSLATION
    Publication number: 20230250417
    Abstract: The present disclosure relates to methods of identifying RNA targets of ribosomes. Some embodiments of the present disclosure relate to a method that can look at multiple subunits and cofactors of the ribosome and the RNA transcripts that are associated with them. Further, kits are disclosed for preparing and producing the methods described herein.
    Type: Application
    Filed: December 29, 2022
    Publication date: August 10, 2023
    Inventors: Daniel A. Lorenz, Maya T. Kunkel, Alexander A. Shishkin, Karen B. Chapman
  • KITS AND METHODS FOR ENRICHMENT OF FULL-LENGTH RNA
    Publication number: 20230137823
    Abstract: Described herein are, at least in part, novel methods for enriching full length RNAs. In aspects of the disclosure, methods for enrichment of RNAs, kits for making such full length RNA reads, and composition for enriching RNAs or cDNA libraries derived therefrom are provided herein. In some embodiments, the method for enriching for full length RNA comprises isolating an RNA sample, contacting the RNA sample with a 5? exonuclease; and performing a reverse transcription reaction to convert the RNA to cDNA.
    Type: Application
    Filed: October 27, 2022
    Publication date: May 4, 2023
    Inventors: DANIEL A. LORENZ, Anide Johansen, Karen B. Chapman
  • METHODS AND USE OF CHIMERIC PROTEINS
    Publication number: 20230138328
    Abstract: Provided are methods for using chimeric proteins to produce RNA modifications that can be detected by sequencing methods, including methods detecting relative translation rates of various mRNAs. Also provided herein are compositions comprising chimeric proteins, wherein the chimeric proteins comprise a RNA editing protein and a ribosomal protein.
    Type: Application
    Filed: March 12, 2021
    Publication date: May 4, 2023
    Inventors: Eugene Yeo, Kristopher W. Brannan, Ryan Marina, Isaac A. Chaim, Daniel A. Lorenz
  • METHODS FOR DETECTING RNA BINDING PROTEIN COMPLEXES
    Publication number: 20230126137
    Abstract: The present disclosure relates to methods of identifying RNA targets of RNA binding proteins. In aspects, the disclosure relates to a method of identifying RNA molecules bound by RNA binding proteins. Some embodiments of the present disclosure relate to a method that can definitively identify direct RNA-target interactions with targeted proteins without the requirement for immunoprecipitation or gel extraction. In some embodiments, the method may include combining multiple antibodies in the same sample.
    Type: Application
    Filed: August 18, 2022
    Publication date: April 27, 2023
    Inventors: Daniel A. Lorenz, Karen B. Chapman