Abstract: The disclosure relates to a coating method for coating a component (e.g. motor vehicle body component) with a coating agent (e.g. paint), that includes steps of Defining a pattern on the component surface of the component to be coated, the pattern being a surface area outlined by a contour, and areal coating of the component surface with the coating agent within the contour, sharp-edged coating of the component surface with a coating agent along at least a portion of the contour of the pattern.

Abstract: The invention is directed to a conjugate having the general formula (I) With AR, MU and L1 as repeating units of a polymer MU is a polymer modifying unit or band gap modifying unit that is evenly or randomly distributed along the polymer main chain, L1 is an aryl or a heteroaryl group evenly or randomly distributed along the polymer, L2 is an aryl or a heteroaryl group located on the ends of the polymer, FL is a fluorescent moiety, G1 and G2 stand for hydrogen, halogen or an antigen recognizing moiety, with the provision than at least one of G1 or G2 is an antigen recognizing moiety, characterized in that AR is connected in the polymer chain via the 2,2? or 3,3? or 5,5? or 6,6? or 7,7? or 8,8? position according to general formula (II)

Abstract: The invention is directed to a conjugate having the general formula (I) Wherein AR and MU are repeating units of a polymer MU is a polymer modifying unit or band gap modifying unit that is evenly or randomly distributed along the polymer main chain, G1 and G2 stand for hydrogen, halogen or an antigen recognizing moiety, with the provision that at least one of G1 or G2 is an antigen recognizing moiety, a is 10 to 100 mol %, b is 0 to 90 mol % c is 1 to 10 000; with the provisio that a+b=100 mol % characterized in that AR is connected in the polymer chain via the 2,2? or 3,3? or 5,5? or 6,6? or 7,7? or 8,8? positions according to general formula (II) Wherein the remaining positions 2,2?; 3,3?; 4,4?; 5,5?; 6,6?; 7,7? and 8,8? are substituted with same or different residues selected from the group consisting of H, SO2CF3, SO2Ra, CF3, CCl3, CN, SO3H, NO2, NRaRbRc+, CHO, CORa, CO2Ra, COCl, CONRaRb, F, Cl, Br, I, Ra, ORa, SRa, OCORa, NRaRb, NHCORa, CCRa, aryl-, heteroaryl-, C6H4ORa or C6H4NRaRb, with

Abstract: The invention is directed to a conjugate having the general formula (I) Wherein AR, MU and MU* are repeating units of a polymer and MU and MU* are polymer modifying units or band gap modifying units which are evenly or randomly distributed along the polymer main chain, G1 and G2 stand for hydrogen, halogen or an antigen recognizing moiety, with the provision that at least one of G1 or G2 is an antigen recognizing moiety, a is 10 to 100 mol %, b is 0 to 90 mol % c is 0.

Abstract: The invention is directed to a method for detecting a target moiety in a sample of biological specimens by providing a conjugate with the general formula (I) characterized in contacting the sample of biological specimens with at least one conjugate (I), thereby labeling the target moiety recognized by an antigen recognizing moiety with the conjugate (I); exciting the labelled target moieties with light having a wavelength within the absorbance spectrum of the fluorescent moiety FL; detecting the labelled target moieties by detecting the fluorescence radiation emitted by the fluorescent moiety FL and degrading the fluorescent moiety FL of the labelled target moieties by irradiating the conjugate with light having a wavelength within the absorbance spectrum of fluorescent moiety FL for a time sufficient to deliver enough energy to reduce the fluorescence radiation emitted by the fluorescent moiety FL at least by 75% of the initial fluorescence radiation.

Abstract: The invention relates to a fluorescent marker according to the following formula I wherein Fluo is selected from the group consisting of substituted or non-substituted C10-C30 acridone, aminoacridine or pyrene, L is a linker and the moiety X is either a —SO2— or a —PO(OH)— moiety. Furthermore, the present invention relates to the use of the fluorescent markers of the invention, in particular also as a standard in glycan analysis, conjugates of fluorescent markers and glycans, as well as a kit-of-parts containing the fluorescent markers of the invention.