Patents by Inventor Daniel Branton

Daniel Branton has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200158712
    Abstract: Provided herein is a nanopore sensor, including a self-supported solid state material selected from hexagonal-BN, a mono-atomic glass, MoS2, WS2, MoSe2, MoTe2, TaSe2, NbSe2, NiTe2, Bi2Sr2CaCu2Ox, and Bi2Te3, having a thickness less than about 5 nm. A nanopore extends through the material thickness. A connection from the first material surface to a first reservoir provides, at the first material surface, a species in an ionic solution from the first reservoir to the nanopore, and a connection from the second material surface to a second reservoir collects in the second reservoir the species and ionic solution after translocation of the species and ionic solution through the nanopore. An electrical circuit is connected with the nanopore, through the material thickness, from the first reservoir to the second reservoir, to monitor translocation of species in the ionic solution through the nanopore in the solid state material.
    Type: Application
    Filed: January 14, 2020
    Publication date: May 21, 2020
    Applicant: President and Fellows of Harvard College
    Inventors: Daniel Branton, Slaven Garaj, Jene A. Golovchenko
  • Patent number: 10564144
    Abstract: There is provided a substantially bare, self-supported single-layer graphene membrane including a nanopore extending through a thickness of the graphene membrane from a first to a second membrane surface opposite the first graphene membrane surface. A connection from the first graphene membrane surface to a first reservoir provides, at the first graphene membrane surface, a species in an ionic solution to the nanopore, and a connection from the second graphene membrane surface to a second reservoir is provided to collect the species and ionic solution after translocation of the species and ionic solution through the nanopore from the first graphene membrane surface to the second graphene membrane surface. An electrical circuit is connected on opposite sides of the nanopore to measure flow of ionic current through the nanopore in the graphene membrane.
    Type: Grant
    Filed: March 13, 2012
    Date of Patent: February 18, 2020
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Slaven Garaj, Jene A. Golovchenko, Daniel Branton
  • Patent number: 10228348
    Abstract: Provided is a solid state support structure including an aperture having a molecular entrance and a molecular exit. A first reservoir is in fluidic communication with the molecular entrance of the aperture and contains a molecule-bearing liquid solution. A second reservoir is in fluidic communication with the molecular exit of the aperture for containing a molecule-bearing liquid solution. A first liquid channel is connected to the first reservoir within less than about 300 microns of the aperture in the support structure and includes molecule-bearing liquid solution for delivery to the first reservoir. A second liquid channel is connected to the second reservoir for accepting molecule-bearing liquid solution from the second reservoir. An electrical connection between the first reservoir and the second reservoir imposes an electrical bias between the first reservoir and the second reservoir for driving the molecule-bearing liquid solution through the aperture in the solid state support structure.
    Type: Grant
    Filed: January 22, 2016
    Date of Patent: March 12, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Daniel Branton, Jene A Golovchenko
  • Patent number: 10202645
    Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: February 12, 2019
    Assignee: The Regents of the University of California
    Inventors: Mark A. Akeson, Daniel Branton, David W. Deamer, Seiko L. Endo
  • Publication number: 20190004029
    Abstract: In a method p for controlling translocation of a target polymer molecule through a nanopore, a clamp is reversibly bound to a sequential plurality of polymer subunits along the target polymer molecule length and the molecule and clamp are disposed in an ionic solution that is in fluidic communication with the nanopore. A constant translocation force is applied across the nanopore to induce travel of the target polymer molecule into the nanopore, until the clamp abuts the nanopore aperture and stops further travel of the target polymer molecule into the nanopore. Then a voltage control pulse is applied across the nanopore and/or a thermal control pulse is applied at the nanopore, with a pulse duration that steps the clamp along the target polymer molecule by no more than one polymer subunit in a direction opposite that of travel into the nanopore. No fuel is provided to the clamp.
    Type: Application
    Filed: June 28, 2018
    Publication date: January 3, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: Daniel Branton, Stephen Jordan Fleming, Jene A. Golovchenko
  • Publication number: 20180023136
    Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
    Type: Application
    Filed: September 22, 2017
    Publication date: January 25, 2018
    Inventors: Mark A. Akeson, Daniel Branton, David W. Deamer, Seico Benner
  • Patent number: 9864775
    Abstract: A user interface is presented that is configured for receiving a user search criteria and search filter limit for a search request. An indicator is presented. Presenting the indicator includes changing appearance of the indicator to indicate increasing strength of the search request.
    Type: Grant
    Filed: March 26, 2015
    Date of Patent: January 9, 2018
    Assignee: International Business Machines Corporation
    Inventors: Daniel A. Branton, Patrick F. Chew, Joseph G. Keller
  • Patent number: 9797863
    Abstract: The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: October 24, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Slaven Garaj, Daniel Branton
  • Publication number: 20170158487
    Abstract: There is provided a nanometric structure that includes a self-supporting nanometric material having a thickness of no more than about 5 nm. A plurality of nanopores is provided in the nanometric material, and the nanopore plurality has a density of at least about 1000 nanopores/cm2. Each nanopore in the plurality of nanopores has a diameter that is no greater than about 10 nm. The plurality of nanopores is monodisperse in diameter with a variation of about ±30%. In a further nanometric structure provided herein there is included a self-supporting nanometric material having a thickness of no more than about 5 nm. A plurality of nanopores in the nanometric material includes at least about 50 nanopores. Each nanopore in the plurality of nanopores has a diameter that is no greater than about 10 nm. The plurality of nanopores is monodisperse in diameter with a variation of about ±30%.
    Type: Application
    Filed: February 17, 2017
    Publication date: June 8, 2017
    Applicant: President and Fellows of Havard College
    Inventors: Christopher John Russo, Jene A. Golovchenko, Daniel Branton
  • Patent number: 9611140
    Abstract: In a method of forming a nanopore in a nanometric material, a nanopore nucleation site is formed at a location that is interior to lateral edges of the nanometric material by directing a first energetic beam, selected from the group of ion beam and neutral atom beam, at the interior location for a first time duration that imposes a first beam dose which causes removal of no more than five interior atoms from the interior location to produce at the interior location a nanopore nucleation site having a plurality of edge atoms. A nanopore is then formed at the nanopore nucleation site by directing a second energetic beam, selected from the group consisting of electron beam, ion beam, and neutral atom beam, at the nanopore nucleation site with a beam energy that removes edge atoms at the nanopore nucleation site but does not remove bulk atoms from the nanometric material.
    Type: Grant
    Filed: March 14, 2012
    Date of Patent: April 4, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Christopher John Russo, Jene Golovchenko, Daniel Branton
  • Publication number: 20160283546
    Abstract: A user interface is presented that is configured for receiving a user search criteria and search filter limit for a search request. An indicator is presented. Presenting the indicator includes changing appearance of the indicator to indicate increasing strength of the search request.
    Type: Application
    Filed: March 26, 2015
    Publication date: September 29, 2016
    Inventors: Daniel A. Branton, Patrick F. Chew, Joseph G. Keller
  • Publication number: 20160139079
    Abstract: Provided is a solid state support structure including an aperture having a molecular entrance and a molecular exit. A first reservoir is in fluidic communication with the molecular entrance of the aperture and contains a molecule-bearing liquid solution. A second reservoir is in fluidic communication with the molecular exit of the aperture for containing a molecule-bearing liquid solution. A first liquid channel is connected to the first reservoir within less than about 300 microns of the aperture in the support structure and includes molecule-bearing liquid solution for delivery to the first reservoir. A second liquid channel is connected to the second reservoir for accepting molecule-bearing liquid solution from the second reservoir. An electrical connection between the first reservoir and the second reservoir imposes an electrical bias between the first reservoir and the second reservoir for driving the molecule-bearing liquid solution through the aperture in the solid state support structure.
    Type: Application
    Filed: January 22, 2016
    Publication date: May 19, 2016
    Applicant: President and Fellows of Harvard College
    Inventors: Daniel Branton, Jene A. Golovchenko
  • Patent number: 9274097
    Abstract: Provided is a first reservoir for containing a liquid solution including a molecule to be characterized and a second reservoir for containing a liquid solution. A solid state support includes a nanopore having a molecular inlet providing a fluidic connection to the first reservoir and a molecular outlet providing a fluidic connection to the second reservoir. An electrical connection is disposed between the first and second reservoirs to apply a molecular translocation voltage across the nanopore between the molecular inlet entrance and outlet exit. At least one electrical probe is disposed at the nanopore to apply a first voltage bias with respect to translocation voltage to slow progression of a molecule through the nanopore between the molecular inlet and outlet and to apply a second voltage bias with respect to translocation voltage to cause the molecule to proceed through the nanopore between the molecular inlet and outlet.
    Type: Grant
    Filed: March 4, 2011
    Date of Patent: March 1, 2016
    Assignee: President and Fellows of Harvard College
    Inventors: Jene A. Golovchenko, Daniel Branton
  • Publication number: 20150307931
    Abstract: The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.
    Type: Application
    Filed: January 9, 2014
    Publication date: October 29, 2015
    Inventors: Mark AKESON, Daniel BRANTON, George CHURCH, David W. DEAMER
  • Patent number: 9046483
    Abstract: The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid.
    Type: Grant
    Filed: August 8, 2013
    Date of Patent: June 2, 2015
    Assignee: President and Fellows of Harvard College
    Inventors: Timothy J. Denison, Alexis Sauer-Budge, Jene A. Golovchenko, Amit Meller, Eric Brandin, Daniel Branton
  • Publication number: 20150101931
    Abstract: The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.
    Type: Application
    Filed: September 5, 2014
    Publication date: April 16, 2015
    Inventors: Slaven GARAJ, Daniel BRANTON
  • Patent number: 8986528
    Abstract: The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: March 24, 2015
    Assignee: President and Fellows of Harvard College
    Inventors: Timothy J. Denison, Alexis Sauer-Budge, Jene A. Golovchenko, Amit Meller, Eric Brandin, Daniel Branton
  • Patent number: 8828211
    Abstract: The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: September 9, 2014
    Assignee: President and Fellows of Harvard College
    Inventors: Slaven Garaj, Daniel Branton
  • Patent number: 8790863
    Abstract: In a method for imaging a solid state substrate, a vapor is condensed to an amorphous solid water condensate layer on a surface of a solid state substrate. Then an image of at least a portion of the substrate surface is produced by scanning an electron beam along the substrate surface through the water condensate layer. The water condensate layer integrity is maintained during electron beam scanning to prevent electron-beam contamination from reaching the substrate during electron beam scanning. Then one or more regions of the layer can be locally removed by directing an electron beam at the regions. A material layer can be deposited on top of the water condensate layer and any substrate surface exposed at the one or more regions, and the water condensate layer and regions of the material layer on top of the layer can be removed, leaving a patterned material layer on the substrate.
    Type: Grant
    Filed: October 26, 2011
    Date of Patent: July 29, 2014
    Assignee: President and Fellows of Harvard College
    Inventors: Daniel Branton, Anpan Han, Jene A. Golovchenko
  • Publication number: 20140079936
    Abstract: In a method of forming a nanopore in a nanometric material, a nanopore nucleation site is formed at a location that is interior to lateral edges of the nanometric material by directing a first energetic beam, selected from the group of ion beam and neutral atom beam, at the interior location for a first time duration that imposes a first beam dose which causes removal of no more than five interior atoms from the interior location to produce at the interior location a nanopore nucleation site having a plurality of edge atoms. A nanopore is then formed at the nanopore nucleation site by directing a second energetic beam, selected from the group consisting of electron beam, ion beam, and neutral atom beam, at the nanopore nucleation site with a beam energy that removes edge atoms at the nanopore nucleation site but does not remove bulk atoms from the nanometric material.
    Type: Application
    Filed: March 14, 2012
    Publication date: March 20, 2014
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Christopher John Russo, Jene Golovchenko, Daniel Branton