Abstract: In one aspect, methods for liver cancer detection are provided that use a combination of genome-wide mutation and fragmentation features of cell free DNA (cfDNA) that facilitate cancer screening. In one aspect, a method of diagnosing liver diseases or disorders in a subject, comprises isolating circulating cfDNA from a subject; conducting whole genome sequencing of cfDNA molecules to generate genomic libraries and fragmentation profiles; comparing the fragmentation profiles to healthy subjects and/or reference genome, and diagnosing whether the subject has a liver disease or disorder.

Abstract: Methods for non-invasive cancer detection use a combination of genome-wide mutation and fragmentation features of cfDNA that facilitate cancer screening.