Abstract: A dilution control system for diluting a first fluid with a second fluid and dispensing diluted fluid is disclosed. The dilution control system includes a bottle that is adapted for receiving a quantity of the first fluid. A valve insert is disposed in the opening of the bottle and includes at least one valve for controlling the flow of the first fluid from the bottle and an air vent. The dilution control system further includes a dispenser assembly having a body with at least one mixing chamber for receiving quantities of the first and second fluids and dispensing the diluted fluid, a platform for engaging and supporting the bottle on the body while dispensing the first fluid into the chamber, and a manifold assembly for dispensing the second fluid into the chamber and for actuating the valve and the air vent of the valve insert. The manifold assembly actuates the valve and the air vent for allowing the first fluid to flow into the mixing chamber only while dispensing the second fluid into the chamber.