Abstract: the subject invention provides novel Plasmodium falciparum antigens and novel polynucleotides encoding these antigens. Also provided by the subject invention are methods of using these antigens and polynucleotides.

Abstract: The inventive subject matter relates to an immunogenic composition and method of enhancing immunogenicity and protective immunity induced by any subunit or whole organism vaccine or combination of vaccines comprising administering to the subject a priming immunization preparation containing an antigen or fragment thereof, said preparation being selected from the group consisting of: a recombinant virus expression system; a recombinant protein antigen or a recombinant polypeptide; a synthetic peptide; a polynucleotide vector; a whole organism or extract and combinations thereof; and a boosting immunization of at least one alphavirus replicon containing an antigen or fragment thereof. The inventive subject matter further relates to an immunogenic composition and method of inducing an immune response that activates both cellular and humoral arms of the immune system.

Abstract: Two proteins and their use as substrates for vaccines intended to initiate an immune response in a mammalian subject against infection with species of Plasmodium for use in the diagnosis of Plasmodium infection and for their use in the development of antimalarial drugs. This invention also relates to the diagnostic, isolation and purification assays based on these Plasmodium proteins. This invention further relates to immunological reagents, specifically antibodies directed against these Plasmodium proteins.