Abstract: [Problem to be Solved by the Invention] The purpose of the present invention is to provide a competitive immunochromatographic analysis method and immunochromatographic sensor using said analysis method capable of intuitively displaying a molecular concentration of a substance to be measured and facilitating visual judgement of the molecular concentration.

Abstract: A fully integrated Òsample-in-signal-out Ó microfluidic paper-based analytical devices are provided relying on bioluminescence resonance energy transfer (BRET) switches for target analyte recognition and colorimetric signal generation. Simultaneous colorimetric detection and quantification of multiple antibodies in a single drop of whole blood is shown. The devices make use of BRET-based antibody sensing proteins integrated into vertically assembled layers of functionalized porous layer(s) of material. The device enables sample volume independent, eliminates addition of reagents to the sample, and has on-device blood plasma separation. User operation is a single drop of a sample and the acquisition of a photograph after sample introduction, with no requirement for precise pipetting, liquid handling or analytical equipment except for a camera. Using different antibodies as targets, simultaneous detection in whole blood was achieved.

Abstract: There is provided a series of coelenterazine (CTZ) derivatives as a substrate with high luminescence intensity, which is optimal for maximum luminescence wavelengths at both 400 nm (blue-shifted RLuc luminescence system) and 500 nm (ALuc luminescence system) for bioassays which is more sensitive than known techniques. The novel CTZ derivatives are compounds in which a specific position(s) of the CTZ is/are substituted with a specific substituent(s) as shown, for example, by the Formula [I], and has a higher luminescence intensity than known CTZ derivatives in blue-shifted RLuc luminescence system or ALuc luminescence system.

Abstract: There is provided a series of coelenterazine (CTZ) derivatives as a substrate with high luminescence intensity, which is optimal for maximum luminescence wavelengths at both 400 nm (blue-shifted RLuc luminescence system) and 500 nm (ALuc luminescence system) for bioassays which is more sensitive than known techniques. The novel CTZ derivatives are compounds in which a specific position(s) of the CTZ is/are substituted with a specific substituent(s) as shown, for example, by the Formula [I], and has a higher luminescence intensity than known CTZ derivatives in blue-shifted RLuc luminescence system or ALuc luminescence system.

Abstract: A novel fluorescent compound having a high light fastness, high fluorescence quantum yield and sharp absorption spectrum, which emits fluorescence having a wavelength in long wavelength region, as well as its use as a labeling agent, is disclosed. In Formula [I] below, by forming a specific hetero ring(s) with R1 and R2, and/or R6 and R7, shift of the wavelength of the fluorescence to longer wavelength and increase in molar extinction coefficient are attained maintaining the high light fastness, high fluorescence quantum yield and sharp absorption spectrum which the fluorescent dyes having the boron dipyrromethene skeleton have.

Abstract: A method for measuring tastes, which can better simulate the human gustation than known methods, as well as a taste sensor, computer program and an apparatus for measuring tastes, is disclosed. In this method, data processing is carried out by a two-phase radial basis function neural network. That is, by sensors, each of which sensors can quantify at least one component representing, individually or cooperatively, the taste of saltiness, sourness, sweetness, umami or bitterness, to obtain a response value from each sensor, and each of the obtained response values is input to a first phase radial basis function neural network to calculate the concentration of each component from each response value.

Abstract: A method for measuring tastes, which can better simulate the human gustation than known methods, as well as a taste sensor, computer program and an apparatus for measuring tastes, is disclosed. In this method, data processing is carried out by a two-phase radial basis function neural network. That is, by sensors, each of which sensors can quantify at least one component representing, individually or cooperatively, the taste of saltiness, sourness, sweetness, umami or bitterness, to obtain a response value from each sensor, and each of the obtained response values is input to a first phase radial basis function neural network to calculate the concentration of each component from each response value.

Abstract: A novel fluorescent compound having a high light fastness, high fluorescence quantum yield and sharp absorption spectrum, which emits fluorescence having a wavelength in long wavelength region, as well as its use as a labeling agent, is disclosed. In Formula [I] below, by forming a specific hetero ring(s) with R1 and R2, and/or R6 and R7, shift of the wavelength of the fluorescence to longer wavelength and increase in molar extinction coefficient are attained maintaining the high light fastness, high fluorescence quantum yield and sharp absorption spectrum which the fluorescent dyes having the boron dipyrromethene skeleton have.