Abstract: A social media computer system includes a database that is in network communication with a first mobile phone and a second mobile phone. The database receives a first set of data from the first mobile phone and a second set of data from the second mobile phone. The database allows the second mobile phone to access the first set of data only after the second mobile phone authorizes the first mobile phone via the database to access the second set of data. A portion of the first set of data accessed by the second mobile phone can be automatically or manually synchronized to the memory of the second mobile phone. A user of the second mobile phone can control the portion of the first set of data synchronized to the second mobile phone. The first set of data can include video, audio, geographic location, visual and textual data. The first mobile phone has a memory, and a portion of the second set of data can be synchronized to the memory of the first mobile phone.

Abstract: A system for transmission of data from a first device operated by a first user includes a wireless device and a database. The wireless device is used by a second user and includes a memory for storage of data. The database is in network communication with the first device and the wireless device. The database receives and stores data from the first device. The database can synchronize a portion of the data from the first device with the memory of the wireless device on a periodic basis that is determined by the second user. The wireless device can include a mobile phone. Data from the first device can include a current mode of access of the first user. The database can synchronize a portion of the data from the first device with the memory of the wireless device automatically according to a predetermined time determined by the second user.

Abstract: A social media computer system and method for transmission of data between a first mobile phone and a second mobile phone includes a database that is configured to be in network communication with the first mobile phone and the second mobile phone. The database is configured to receive a first set of data input by the first mobile phone and a second set of data input by the second mobile phone. The database is configured to allow the second mobile phone to access at least a portion of the first set of data only after the second mobile phone authorizes the first mobile phone via the database to access the second set of data. The database can include a plurality of privacy levels that are alternately selectable by the first mobile phone to control the amount of the first set of data that is accessible by the second mobile phone.

Abstract: A system for transmission of data from a first device operated by a first user includes a wireless device and a database. The wireless device is used by a second user and includes a memory for storage of data. The database is in network communication with the first device and the wireless device. The database receives and stores data from the first device. The database can synchronize a portion of the data from the first device with the memory of the wireless device on a periodic basis that is determined by the second user. The wireless device can include a mobile phone. Data from the first device can include a current mode of access of the first user. The database can synchronize a portion of the data from the first device with the memory of the wireless device automatically according to a predetermined time determined by the second user.

Abstract: A system for transmission of data between a first device operated by a first user and a second device operated by a second user includes a database that is in network communication with the first device and the second device. The database receives a first set of data input by the first user and a second set of data input by the second user. The database allows the second user to access a first portion of the first set of data only after the second user authorizes the first user via the database to access a first portion of the second set of data. Additionally, the database can allow the second user to have access to the first portion of the first set of data from the database only after the first user authorizes the second user via the database to access the first portion of the first set of data from the database.

Abstract: A system for transmission of data from a first device operated by a first user includes a wireless device and a database. The wireless device is used by a second user and includes a memory for storage of data. The database is in network communication with the first device and the wireless device. The database receives and stores data from the first device. The database can synchronize a portion of the data from the first device with the memory of the wireless device on a periodic basis that is determined by the second user. The wireless device can include a mobile phone. Data from the first device can include a current mode of access of the first user. The database can synchronize a portion of the data from the first device with the memory of the wireless device automatically according to a predetermined time determined by the second user.

Abstract: A system for transmission of data between a first device operated by a first user and a second device includes a database that receives a first set of data input by the first user and a second set of data input by the second user. In one embodiment, the first set of data includes an immediate mode of access and/or one or more future modes of access of the first user which correlate to one or more specific time periods during which the future mode of access will become the immediate mode of access. Additionally, the first set of data can include a time-dependent schedule of the future mode of access of the first user.

Abstract: A system for transmission of mode of access information between a first device operated by a first user and a second device operated by a second user includes a database that is in network communication with the first device and the second device. The database stores a plurality of different modes of access for contacting the first user that are devoid of alpha-numeric identifiers specific to the first user. The database allows the first user to select one of the modes of access as a current mode of access of the first user. The current mode of access of the first user can be communicated to the second device once the second device queries the database. The current mode of access of the first user can be selected from the group consisting of mobile phone, text message and electronic mail. The current mode of access of the first user can change automatically over time according to a schedule input into the database by the first user.

Abstract: The present invention provides methods for analyzing exon expression profiles of a cell or type of cell. In the invention, the expression levels of a plurality of individual exons or multiexons for each of a plurality of genes in the genome of an organism are measured and analyzed to determine the biological state, such as the exon expression state or transcriptional state, of the cell or type of cell. The methods of the invention are useful for determination of alternative RNA splicing in a plurality of genes. The invention also provides nucleic acid probe arrays for determining in parallel the expression levels of a plurality of exons or multiexons for each of a plurality of genes in the genome of an organism. The invention further provides methods for determining the effects of perturbations, such as perturbations by drugs, on exon expression and alternative RNA splicing pathways.

Abstract: A method for detecting the presence of a nucleic acid template (110) in a sample includes the steps of combining the sample in a reaction vessel with a first primer (112F) and a second primer (112R) having a first section (114), a second section (118) and a spacer (116). The method also includes one or more of the steps of extending the first section (114) with additional nucleotides, binding the first primer (112F) to the extended first section, extending the first primer (112F) with additional nucleotides and terminating extension of the first primer (112F) with the spacer (116). The first section (114) includes a plurality of nucleotides that bind with a portion of the nucleic acid template (110). The second section (118) is spaced-apart from the first section (114) and includes a plurality of nucleotides that do not bind with the nucleic acid template (110). The spacer (116) couples the first section (114) to the second section (118). The invention is also directed toward the second primer (112R).

Abstract: Methods for amplification and capture of nucleic acid sequences include annealing a forward primer to a DNA or RNA template in a first reaction vessel including fewer than four different dNTPs; forming an extended primer that terminates when an omitted dNTP is required for further extension; releasing the extended primer; exponentially amplifying the extended primer in a second reaction vessel that includes a reverse primer, four different dNTPs and a capture probe that includes n oligonucleotides having fewer than n locking nucleic acids; and concurrently capturing one of the extended primers with the capture probe while amplifying the extended primer. The steps of annealing, extending and releasing can occur at a first reaction temperature that is substantially isothermal and in the absence of a helicase. The steps of exponentially amplifying and capturing can occur at a second reaction temperature that is substantially isothermal.

Abstract: A method for detecting the presence of a nucleic acid template (110) in a sample includes the steps of combining the sample in a reaction vessel with a first primer (112F) and a second primer (112R) having a first section (114), a second section (118) and a spacer (116). The method also includes one or more of the steps of extending the first section (114) with additional nucleotides, binding the first primer (112F) to the extended first section, extending the first primer (112F) with additional nucleotides and terminating extension of the first primer (112F) with the spacer (116). The first section (114) includes a plurality of nucleotides that bind with a portion of the nucleic acid template (110). The second section (118) is spaced-apart from the first section (114) and includes a plurality of nucleotides that do not bind with the nucleic acid template (110). The spacer (116) couples the first section (114) to the second section (118). The invention is also directed toward the second primer (112R).

Abstract: A method for amplifying a nucleic acid sequence includes the steps of (i) providing a first pair of primers that include one or more uracil nucleotides, the primers being complementary to a portion of a genomic template, (ii) introducing the first pair of primers, the genomic template and a first polymerase into a reaction vessel, (iii) carrying out one or more polymerase chain reaction cycles in the reaction vessel to generate a plurality of first amplicons, and (iv) selectively degrading a portion each first amplicon with a Uracil-DNA Glycosylase to decrease the binding energy of each first amplicon. In one embodiment, the step of selectively degrading includes using a thermostable Uracil-DNA Glycosylase to decrease the binding energy of each first amplicon. In another embodiment, the method also includes the step of adding a second polymerase and a second pair of primers to the reaction vessel to generate a plurality of second amplicons that are different than the first amplicons.

Abstract: In one aspect the present invention provides methods of synthesizing a preparation of nucleic acid molecules, the methods comprising the steps of: (a) utilizing an RNA template to enzymatically synthesize a first DNA molecule that is complementary to at least 50 contiguous bases of the RNA template; (b) utilizing the first DNA molecule as a template to enzymatically synthesize a second DNA molecule, thereby forming a double-stranded DNA molecule wherein the first DNA molecule is hybridized to the second DNA molecule; (c) utilizing the first or second DNA molecule of the double-stranded DNA molecule as a template to enzymatically synthesize a first RNA molecule that is complementary to either the first DNA molecule or to the second DNA molecule; and (d) utilizing the first RNA molecule as a template to enzymatically synthesize a third DNA molecule that is complementary to the first RNA molecule.

Abstract: A method for amplification and capture of nucleic acid sequences can include the steps of annealing a forward primer to a DNA or RNA template in a first reaction vessel that includes fewer than four different dNTPs; extending the forward primer with the dNTPs to form an extended primer that terminates when an omitted dNTP is required for further extension of the forward primer; releasing the extended primer; exponentially amplifying the extended primer in a second reaction vessel that includes a reverse primer, four different dNTPs and a capture probe, the capture probe including n oligonucleotides, wherein fewer than n of the oligonucleotides are locking nucleic acids; and concurrently capturing one of the extended primers in the second reaction vessel with the capture probe while amplifying the extended primer. Further, in certain embodiments, the steps of annealing, extending and releasing occur at a first reaction temperature that is substantially isothermal and in the absence of a helicase.

Abstract: A system for transmission of data from a first device operated by a first user includes a wireless device and a database. The wireless device is used by a second user and includes a memory for storage of data. The database is in network communication with the first device and the wireless device. The database receives and stores data from the first device. The database can synchronize a portion of the data from the first device with the memory of the wireless device on a periodic basis that is determined by the second user. The wireless device can include a mobile phone. Data from the first device can include a current mode of access of the first user. The database can synchronize a portion of the data from the first device with the memory of the wireless device automatically according to a predetermined time determined by the second user.

Abstract: A system for transmission of mode of access information between a first device operated by a first user and a second device operated by a second user includes a database that is in network communication with the first device and the second device. The database stores a plurality of different modes of access for contacting the first user that are devoid of alpha-numeric identifiers specific to the first user. The database allows the first user to select one of the modes of access as a current mode of access of the first user. The current mode of access of the first user can be communicated to the second device once the second device queries the database. The current mode of access of the first user can be selected from the group consisting of mobile phone, text message and electronic mail. The current mode of access of the first user can change automatically over time according to a schedule input into the database by the first user.

Abstract: The invention relates to methods and systems (e.g., computer systems and computer program products) for identifying and characterizing genes using microarrays. In particular, the invention provides for improved, robust methods for detecting genes through the use of microarrays to analyze the expression state of the genome. Genes which are expressed can be mapped to their respective positions in the genome, and the structure of such genes can be determined.

Abstract: Methods of selecting tag nucleic acids and VLSIPS™ arrays and the arrays made by the methods are used to label and track compositions, including cells and viruses, e.g., in libraries of cells or viruses. In addition to providing a way of tracking compositions in mixtures, the tags facilitate analysis of cell and viral phenotypes.

Abstract: The present invention relates to methods of using synthetic lethal screening techniques to identify drug targets. The methods of the present invention use “barcoded” libraries of cells, where the library consists of a collection of different mutant clones, each mutant clone bearing a knock-out mutation of a different gene. Each mutant clone has a unique DNA identifier tag, or “barcode,” to allow for quick and convenient identification of the clone and its mutation. The use of such a library allows for rapid, quantitative, sensitive and simple identification of genes which interact with a mutated target gene. So identified genes are promising targets for drug screening.